High rates of photobiological H2 production by a cyanobacterium under aerobic conditions
High rates of photobiological H2 production by a cyanobacterium under aerobic conditions
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DOI：
10.1038/ncomms1139
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发表时间：
2010-12-01
影响因子：
16.6
通讯作者：
Pakrasi, Himadri B.
中科院分区：
文献类型：
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作者：
Bandyopadhyay, Anindita;Stoeckel, Jana;Pakrasi, Himadri B.
在新兴的可再生能源和绿色能源中，生物氢是一个有吸引力的选择。某些具有功能固氮酶和/或双向氢酶的微生物可以产生氢气。特别是，产氧光合作用微生物光生物制氢的潜力引起了人们的极大兴趣。然而，固氮酶和氢酶通常对氧敏感，需要保护机制才能在有氧的细胞外环境中发挥作用。在这里，我们描述了蓝藻。ATCC 51142是一种单细胞、重氮营养的蓝藻，能够在好氧条件下产生高水平的氢气。野生型蓝藻51142在甘油存在下，每毫克叶绿素每小时产氢速率高达465mmol.该菌株的产氢是由一个高效的固氮酶系统介导的，与之前描述的任何野生型产氢光合菌相比，该系统可以被操纵以将太阳能转化为氢的速率提高几倍。
Among the emerging renewable and green energy sources, biohydrogen stands out as an appealing choice. Hydrogen can be produced by certain groups of microorganisms that possess functional nitrogenase and/or bidirectional hydrogenases. In particular, the potential of photobiological hydrogen production by oxygenic photosynthetic microbes has attracted significant interest. However, nitrogenase and hydrogenase are generally oxygen sensitive, and require protective mechanisms to function in an aerobic extracellular environment. Here, we describe Cyanothece sp. ATCC 51142, a unicellular, diazotrophic cyanobacterium with the capacity to generate high levels of hydrogen under aerobic conditions. Wild-type Cyanothece 51142 can produce hydrogen at rates as high as 465 mu mol per mg of chlorophyll per hour in the presence of glycerol. Hydrogen production in this strain is mediated by an efficient nitrogenase system, which can be manipulated to convert solar energy into hydrogen at rates that are several fold higher, compared with any previously described wild-type hydrogen-producing photosynthetic microbe.