BUB-1 targets PP2A:B56 to regulate chromosome congression during meiosis I in C. elegans oocytes.
BUB-1 targets PP2A:B56 to regulate chromosome congression during meiosis I in C. elegans oocytes.
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DOI：
10.7554/elife.65307
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发表时间：
2020-12-23
期刊：
影响因子：
7.7
通讯作者：
Pelisch F
中科院分区：
文献类型：
--
作者：
Bel Borja L;Soubigou F;Taylor SJP;Fraguas Bringas C;Budrewicz J;Lara-Gonzalez P;Sorensen Turpin CG;Bembenek JN;Cheerambathur DK;Pelisch F
蛋白磷酸酶2A（PP 2A）是由支架（A）、催化（C）和调节（B）亚基组成的异源三聚体。PP 2A与B56亚基的复合物被Shugoshin和BUBR 1靶向，以保护着丝粒凝聚力并稳定酵母和小鼠减数分裂中的着丝粒微管附着。在秀丽隐杆线虫中，最接近的BUBR 1直向同源物缺乏B56相互作用结构域，并且Shugoshin不需要减数分裂分离。因此，PP 2A在C.秀丽隐杆线虫雌性减数分裂是未知的。我们报道PP 2A在C.雌性减数分裂。BUB-1是B56亚基在前中期I的主要染色体靶向因子。BUB-1通过新鉴定的LxxIxE基序以磷酸化依赖的方式将PP 2A：B56募集到染色体上，并且这种募集对于正确的染色体聚集是重要的。我们的研究结果强调了B56募集的一种新机制，这对于募集参与减数分裂I期间染色体聚集的PP 2A池至关重要。
Protein Phosphatase 2A (PP2A) is a heterotrimer composed of scaffolding (A), catalytic (C), and regulatory (B) subunits. PP2A complexes with B56 subunits are targeted by Shugoshin and BUBR1 to protect centromeric cohesion and stabilise kinetochore–microtubule attachments in yeast and mouse meiosis. In Caenorhabditis elegans, the closest BUBR1 orthologue lacks the B56-interaction domain and Shugoshin is not required for meiotic segregation. Therefore, the role of PP2A in C. elegans female meiosis is unknown. We report that PP2A is essential for meiotic spindle assembly and chromosome dynamics during C. elegans female meiosis. BUB-1 is the main chromosome-targeting factor for B56 subunits during prometaphase I. BUB-1 recruits PP2A:B56 to the chromosomes via a newly identified LxxIxE motif in a phosphorylation-dependent manner, and this recruitment is important for proper chromosome congression. Our results highlight a novel mechanism for B56 recruitment, essential for recruiting a pool of PP2A involved in chromosome congression during meiosis I.
影响因子：
2.4
作者：
Lange KI;Heinrichs J;Cheung K;Srayko M
通讯作者：
Srayko M