Biodegradable porous sheet-like scaffolds for soft-tissue engineering using a combined particulate leaching of salt particles and magnetic sugar particles
Biodegradable porous sheet-like scaffolds for soft-tissue engineering using a combined particulate leaching of salt particles and magnetic sugar particles
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DOI：
10.1016/j.jbiosc.2013.01.011
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发表时间：
2013-07-01
影响因子：
2.8
通讯作者：
Arai, Fumihito
中科院分区：
文献类型：
--
作者：
Hu, Chengzhi;Tercero, Carlos;Arai, Fumihito
作为人工细胞外基质（ECM）的支架通过为渗透、向内生长和血管化提供最佳的细胞环境在组织再生过程中发挥关键作用。堆叠的片状支架可以被改造成人工ECM，这表明实现复杂的3D组织再生以支持细胞存活和生长的巨大潜力。在这项研究中，我们提出并研究了磁性糖颗粒（MSPs）和盐颗粒的组合颗粒沥滤，用于开发片状支架。MSP通过将NdFeB颗粒封装在糖球内来制造，并且在制造支架时使用磁场作为致孔剂来控制孔径、孔结构和孔密度。我们研究了在片状支架的制备过程中，磁场强度对控制未磁化的MSP涂层厚度的影响。给出了磁感应强度与MSP层厚度之间的实验关系。此外，我们研究了不同浓度的聚（L-丙交酯-co-ε-己内酯）（PLCL）作为支架材料对MSP簇的渗透能力。在聚合物浇铸和去除糖模板之后，在支架内产生球形孔。NIH/3 T3成纤维细胞在该支架上的培养证明了该方法可以应用于细胞片的制备。(C)2013年，生物技术学会，日本。All rights reserved.
Scaffolds serving as artificial extracellular matrixes (ECMs) play a pivotal role in the process of tissue regeneration by providing optimal cellular environments for penetration, ingrowth, and vascularization. Stacks of sheet-like scaffold can be engineered to become artificial ECMs, suggesting a great potential for achieving complex 3-D tissue regeneration to support cell survival and growth. In this study, we proposed and investigated a combined particulate leaching of magnetic sugar particles (MSPs) and salt particles for the development of a sheet-like scaffold. MSPs were fabricated by encapsulating NdFeB particles inside sugar spheres and were controlled using magnetic fields as a porogen to control pore size, pore structure and pore density while fabricating the scaffold. We studied the influence of the strength of the magnetic fields in controlling the coating thickness of the unmagnetized MSPs during the fabrication of the sheet-like scaffolds. The experimental relationship between magnetic flux density and the thickness of the MSP layer was illustrated. Furthermore, we investigated the infiltration capacity of different concentrations of poly(L-lactide-co-epsilon-caprolactone) (PLCL) as a scaffold material on MSP clusters. Following polymer casting and removal of the sugar template, spherical pores were generated inside the scaffolds. Cultivation of NIH/3T3 fibroblasts on the fabricated scaffold proves that the proposed method can be applied in the cell sheet fabrication. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.