Structure and dynamics of the iron responsive element RNA: Implications for binding of the RNA by iron regulatory binding proteins
Structure and dynamics of the iron responsive element RNA: Implications for binding of the RNA by iron regulatory binding proteins
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DOI：
10.1006/jmbi.1997.1377
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发表时间：
1997-11-21
影响因子：
5.6
通讯作者：
Pardi, A
中科院分区：
文献类型：
--
作者：
Addess, KJ;Basilion, JP;Pardi, A
铁响应元件（IRE）是一个类似于30个核苷酸的RNA发夹，位于所有铁蛋白mRNA的5'非翻译区和所有转铁蛋白受体mRNA的3'非翻译区。IRE与两种相关的IRE结合蛋白（IRP）结合，IRP通过调节铁蛋白和转铁蛋白受体基因的表达来帮助控制细胞内铁水平。多维NMR和计算方法被用来研究的IRE RNA在溶液中的结构和动力学。NMR数据与A型螺旋茎区域的形成、一个碱基的内部凸起和环中第一个和第五个核苷酸之间的Watson-Crick C.G碱基对一致。精细结构的叠加表明，保守的C在内部凸起，和三个残基在六个核苷酸的发夹环是相当动态的RNA。讨论了茎、环和凸起在IRE RNA功能中以及与铁调节蛋白可能相互作用中的结构作用。(C)出版社：Academic Press Limited。
The iron responsive element (IRE) is a similar to 30 nucleotide RNA hairpin that is located in the 5' untranslated region of all ferritin mRNAs and in the 3' untranslated region of all transferrin receptor mRNAs. The IREs are bound by two related IRE-binding proteins (IRPs) which help control intracellular levels of iron by regulating the expression of both ferritin and transferrin receptor genes. Multi-dimensional NMR and computational approaches were used to study the structure and dynamics of the IRE RNA in solution. The NMR data are consistent with formation of A-form helical stem regions, a one-base internal bulge and a Watson-Crick C.G base-pair between the first and fifth nucleotides in the loop. A superposition of refined structures indicates that the conserved C in the internal bulge, and three residues in the six-nucleotide hairpin loop are quite dynamic in this RNA. The structural roles of the stems, the loop and the bulge in the function of the IRE RNA and in possible interactions with the iron regulatory protein are discussed. (C) 1997 Academic Press Limited.