Repeatability of adaptive traits among ethnic Tibetan highlanders
Repeatability of adaptive traits among ethnic Tibetan highlanders
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DOI：
10.1002/ajhb.23670
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发表时间：
2021-08-23
影响因子：
2.9
通讯作者：
Basnyat, Buddha
中科院分区：
文献类型：
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作者：
Beall, Cynthia M.;Childs, Geoff;Basnyat, Buddha
目标将性状与生物学途径和基因联系起来依赖于稳定的观察。研究人员通常只确定一次特征，期望仔细的研究协议产生无噪声的测量结果。本报告通过对高海拔低氧适应性研究中经常使用的特征进行重测重复性分析来研究这种期望，通常是在没有气候控制的情况下。方法291名藏族妇女居住在3500至4200米的上木斯塘区，尼泊尔，提供了三个观察血红蛋白浓度，血红蛋白的氧饱和度的百分比，并通过无创脉搏血氧饱和度的条件下，旨在尽量减少环境噪声的脉搏。结果高的组内相关系数和低的受试者内变异系数反映了测量结果的一致性。血氧饱和度百分比具有最高的组内相关系数和最小的受试者内变异系数;测量噪声主要发生在较低的值中。血红蛋白浓度和脉搏呈现出略高的受试者内变异系数;测量噪声发生在整个值范围内。这些女性在7年前使用相同的设备和协议进行了相同的测量。7年间观察到的样本平均值和SD差异很小。绝经后血红蛋白浓度显著增加。结论分析性状的重复性特征可以改善我们对统计分析的解释和对测量或生物学变异的检测。高水平的测量可重复性和生物稳定性支持继续使用这些强大的特征来研究人类在这一海拔范围内的适应性。
Objectives Connecting traits to biological pathways and genes relies on stable observations. Researchers typically determine traits once, expecting careful study protocols to yield measurements free of noise. This report examines that expectation with test-retest repeatability analyses for traits used regularly in research on adaptation to high-altitude hypoxia, often in settings without climate control. Methods Two hundred ninety-one ethnic Tibetan women residing from 3500 to 4200 m in Upper Mustang District, Nepal, provided three observations of hemoglobin concentration, percent of oxygen saturation of hemoglobin, and pulse by noninvasive pulse oximetry under conditions designed to minimize environmental noise. Results High-intraclass correlation coefficients and low within-subject coefficients of variation reflected consistent measurements. Percent of oxygen saturation had the highest intraclass correlation coefficient and the smallest within-subject coefficient of variability; measurement noise occurred mainly in the lower values. Hemoglobin concentration and pulse presented slightly higher within-subject coefficients of variation; measurement noise occurred across the range of values. The women had performed the same measurements 7 years earlier using the same devices and protocol. The sample means and SD observed across 7 years differed little. Hemoglobin concentration increased substantially after menopause. Conclusions Analyzing repeatability features of traits may improve our interpretation of statistical analyses and detection of variation from measurement or biology. The high levels of measurement repeatability and biological stability support the continued use of these robust traits for investigating human adaptation in this altitude range.