Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: Implications in receptor solubilization
Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: Implications in receptor solubilization
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DOI：
10.1016/0014-5793(96)00733-8
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发表时间：
1996-08-05
期刊：
影响因子：
3.5
通讯作者：
Harikumar, KG
中科院分区：
文献类型：
--
作者：
Chattopadhyay, A;Harikumar, KG
两性离子去污剂 3-[(3-胆酰胺丙基)-二甲基铵]-1-丙磺酸盐 (CHAPS) 性质温和、非变性，广泛用于溶解膜蛋白和受体。我们在此报告 CHAPS 的临界胶束浓度 (CMC) 取决于溶液中 NaCl 的浓度。因此，CHAPS 的 CMC 从不存在任何盐的情况下的 6.41 mM 降低到存在 1.5 M NaCl 的情况下的 4.10 mM。 CMC 值的对数似乎与盐浓度具有线性关系。 CMC 随离子强度的变化对于膜结合神经元受体的溶解具有重要意义。这通过高盐条件下预胶束浓度的 CHAPS 对牛脑海马粗（天然）膜的 1A 型血清素受体 (5-HT1A) 的最佳溶解表明了这一点。
The zwitterionic detergent, 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS), is mild, non-denaturing, and extensively used for solubilizing membrane proteins and receptors. We report here that the critical micelle concentration (CMC) of CHAPS is dependent on the concentration of NaCl in the solution. Thus, the CMC of CHAPS decreases from 6.41 mM in absence of any salt to 4.10 mM in presence of 1.5 M NaCl. The logarithm of the CMC values appear to have a linear relationship with the salt concentration. Such changes in CMC with ionic strength have important implications in solubilization of membrane-bound neuronal receptors. This is shown by optimal solubilization of the serotonin receptor type 1A (5-HT1A) from bovine brain hippocampal crude (native) membrane by CHAPS at pre-micellar concentration under high salt conditions.