A Systems-Based Dissection of Retinal Inputs to the Zebrafish Tectum Reveals Different Rules for Different Functional Classes during Development
A Systems-Based Dissection of Retinal Inputs to the Zebrafish Tectum Reveals Different Rules for Different Functional Classes during Development
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DOI：
10.1523/jneurosci.1866-13.2013
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发表时间：
2013-08-28
影响因子：
5.3
通讯作者：
Meyer, Martin P.
中科院分区：
文献类型：
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作者：
Lowe, Andrew S.;Nikolaou, Nikolas;Meyer, Martin P.
我们研究了斑马鱼视顶盖在发育过程中视网膜输入的三个功能类别的形式、多样性和组织。我们基于系统的方法是分析用突触前靶向钙指示剂突触素GCaMP3(SyGCaMP3)标记的视网膜神经节细胞群的数据。总而言之，我们的发现提供了一个关于视觉编码在视网膜顶盖发育过程中的程度的洞察，以及它是如何从一个新生的和嘈杂的突触前神经场景动态演变为一个日益复杂和精致的表征的。我们报告了五个关键特征：(1)方向选择输入在发育上是不变的；(2)方向选择输入在同一时期表现出高度动态的性质，其功能特征和空间组织发生了变化；(3)被定义为各向异性的输入是早期的优势功能类别，具有异质性的反应轮廓，其发生率和空间范围逐渐减小；(4)黑暗教养选择性地影响方向选择反应：功能特征和相对空间分布；以及(5)方向选择输入表现出四种亚型，比以前在任何物种中发现的多两种。我们的方法是用活动指示器标记RGC轴突终末，并定量表征对不同视觉刺激的一致反应特性。由于斑马鱼的体积小，顶盖容易接触，它在斑马鱼中的应用使对多种功能反应群体的快速而有力的评估成为可能。
We have examined the form, diversity, and organization of three functional classes of retinal inputs to the zebrafish optic tectum during development. Our systems-based approach was to analyze data from populations of retinal ganglion cells labeled with a presynaptic targeted calcium indicator, synaptophysin GCaMP3 (SyGCaMP3). Collectively, our findings provide an insight as to the degree of visual encoding during retino-tectal development and how it dynamically evolves from a nascent and noisy presynaptic neural-scape to an increasingly complex and refined representation. We report five key features: (1) direction-selective inputs are developmentally invariant; (2) orientation-selective inputs exhibit highly dynamic properties over the same period, with changes in their functional characteristics and spatial organization; (3) inputs defined as anisotropic are an early dominant functional class, with heterogeneous response profiles, which progressively diminish in incidence and spatial extent; (4) dark rearing selectively affects the orientation-selective responses: both functional characteristics and relative spatial distributions; and (5) orientation-selective inputs exhibit four subtypes, two more than previously identified in any species. Our approach was to label RGC axon terminals with an indicator of activity and quantitatively characterize coherent response properties to different visual stimuli. Its application in the zebrafish, given its small size and the accessibility of the tectum, has enabled a quick yet robust assessment of multiple functional populations of responses.