The cytotoxic effects of a novel IH636 grape seed proanthocyanidin extract on cultured human cancer cells
The cytotoxic effects of a novel IH636 grape seed proanthocyanidin extract on cultured human cancer cells
复制标题
DOI：
10.1023/a:1006926414683
复制
发表时间：
1999-06-01
影响因子：
4.3
通讯作者：
Bagchi, D
中科院分区：
文献类型：
--
作者：
Ye, X;Krohn, RL;Bagchi, D
葡萄籽原花青素是一种天然抗氧化剂，具有广谱的抗自由基和氧化应激的化学保护作用。在这项研究中，我们已经评估了一种新的葡萄籽原花青素提取物（GSPE）的细胞毒性对MCF-7人乳腺癌细胞，A-427人肺癌细胞，CRL-1739人胃腺癌细胞和K562慢性髓性白血病细胞在25和50毫克/升的浓度为0-72小时使用细胞形态学和MTT细胞毒性试验。此外，我们还比较了对正常人胃粘膜细胞和正常J774A.1小鼠巨噬细胞的影响与对癌细胞系的影响。GSPE对MCF-7乳腺癌、A-427肺癌和胃腺癌细胞的细胞毒作用呈浓度和时间依赖性。MCF-7细胞与25 mg/L GSPE孵育后，在孵育24、48和72 h时分别观察到约6.5%、30%和43%的细胞生长抑制，而MCF-7细胞与50 mg/L GSPE孵育后，在这些相同时间点分别观察到11%、35%和47%的细胞生长抑制。在A-427和胃腺癌细胞中观察到类似的结果。GSPE对K562白血病细胞无细胞毒作用。但GSPE对正常人胃粘膜细胞和J774A.1小鼠巨噬细胞的生长和活力有促进作用。这些数据表明，GSPE表现出对一些癌细胞的细胞毒性，同时增强所检查的正常细胞的生长和活力。
Grape seed proanthocyanidins are natural antioxidants which possess a broad spectrum of chemoprotective properties against free radicals and oxidative stress. In this study, we have assessed the cytotoxicity of a novel IH636 grape seed proanthocyanidin extract (GSPE) against MCF-7 human breast cancer cells, A-427 human lung cancer cells, CRL-1739 human gastric adenocarcinoma cells and K562 chronic myelogenous leukemic cells at 25 and 50 mg/lit concentrations for 0-72 h using cytomorphology and MTT cytotoxicity assay. In addition, we compared the effects on normal human gastric mucosal cells and normal J774A.1 murine macrophage cells with the effects on the cancer cell lines. Concentration- and time-dependent cytotoxic effects of GSPE were observed on the MCF-7 breast cancer, A-427 lung cancer and gastric adenocarcinoma cells. Following incubation of the MCF-7 cells with 25 mg/lit of the GSPE approximately 6.5, 30 and 43% inhibitions in cell growth were observed at 24, 48 and 72 h of incubation, respectively, while incubation of the MCF-7 cells with 50 mg/lit of the GSPE resulted in 11, 35 and 47% inhibition in cell growth at these same points, respectively. Similar results were observed in the A-427 and gastric adenocarcinoma cells. GSPE exhibited no cytotoxicity toward the neoplastic K562 myelogenous leukemic cells. However, GSPE enhanced the growth and viability of the normal human gastric mucosal cells and J774A.1 murine macrophage cells. These data demonstrate that GSPE exhibited cytotoxicity towards some cancer cells, while enhancing the growth and viability of the normal cells which were examined.