Predominant Effect of Host Genetics on Levels of Lactobacillus johnsonii Bacteria in the Mouse Gut
Predominant Effect of Host Genetics on Levels of Lactobacillus johnsonii Bacteria in the Mouse Gut
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DOI：
10.1128/aem.00324-11
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发表时间：
2011-09-01
影响因子：
4.4
通讯作者：
Kashi, Yechezkel
中科院分区：
文献类型：
--
作者：
Buhnik-Rosenblau, Keren;Danin-Poleg, Yael;Kashi, Yechezkel
肠道微生物群与宿主的健康密切相关。其组成受环境因素的影响，如食物和母体接种，而宿主遗传学的相对影响最近被发现。在这里，我们研究了宿主遗传背景对小鼠模型中肠道细菌组成的影响，重点是乳酸菌（LAB）作为一个重要的群体，包括许多益生菌菌株。基于16 S rRNA基因分型，在BALB/c和C57 BL/6 J小鼠系的粪便LAB群体中观察到变异。与BALB/c小鼠粪便相比，C57 BL/6 J中的约氏乳杆菌（一种潜在的益生菌）水平显著更高。在BALB/c小鼠肠道中，L.口服给药后Johnsonii水平迅速下降，表明某些选择力不允许其在较高水平下持续存在。对L.在两个小鼠品系之间的正反交中进一步测试Johnsonii水平。所得F1后代呈现相似的L。johnsonii水平，证实了小鼠遗传学在确定这些水平方面起着重要作用，而母体效应较小。我们的研究结果表明，小鼠遗传学有一个主要的影响，在一般的LAB人口的组成和持久的L。尤其是肠道中的约翰逊氏菌。专注于窄谱的可培养LAB使得能够分离和表征这种潜在的益生菌菌株，其可以作为个性化医学方法的一部分而专门针对宿主的遗传背景。
The gut microbiota is strongly associated with the well-being of the host. Its composition is affected by environmental factors, such as food and maternal inoculation, while the relative impact of the host's genetics have been recently uncovered. Here, we studied the effect of the host genetic background on the composition of intestinal bacteria in a murine model, focusing on lactic acid bacteria (LAB) as an important group that includes many probiotic strains. Based on 16S rRNA gene genotyping, variation was observed in fecal LAB populations of BALB/c and C57BL/6J mouse lines. Lactobacillus johnsonii, a potentially probiotic bacterium, appeared at significantly higher levels in C57BL/6J versus BALB/c mouse feces. In the BALB/c gut, the L. johnsonii level decreased rapidly after oral administration, suggesting that some selective force does not allow its persistence at higher levels. The genetic inheritance of L. johnsonii levels was further tested in reciprocal crosses between the two mouse lines. The resultant F1 offspring presented similar L. johnsonii levels, confirming that mouse genetics plays a major role in determining these levels compared to the smaller maternal effect. Our findings suggest that mouse genetics has a major effect on the composition of the LAB population in general and on the persistence of L. johnsonii in the gut in particular. Concentrating on a narrow spectrum of culturable LAB enables the isolation and characterization of such potentially probiotic bacterial strains, which might be specifically oriented to the genetic background of the host as part of a personalized-medicine approach.