Long-term treatment with green tea polyphenols modifies the gut microbiome of female sprague-dawley rats.
Long-term treatment with green tea polyphenols modifies the gut microbiome of female sprague-dawley rats.
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DOI：
10.1016/j.jnutbio.2018.01.005
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Wang JS
中科院分区：
文献类型：
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作者：
Wang J;Tang L;Zhou H;Zhou J;Glenn TC;Shen CL;Wang JS
绿色茶多酚（GTP）已被证明对动物和人类的健康有益。GTP的有益作用似乎是其与肠道微生物群相互作用的结果。这项研究评估了长期GTP治疗对实验大鼠肠道微生物群的影响，以及肠道微生物群变化与GTP有益作用之间的潜在联系。将6月龄Sprague-Dawley大鼠随机分配至3种给药方案（0、0.5%和1.5% GTP），并随访6个月。在第3个月或第6个月结束时，将每组的一半动物处死，并收集其结肠内容物，用于使用16 S核糖体RNA和鸟枪宏基因组群落测序进行微生物组分析。GTP治疗显着降低了生物多样性和修改的微生物群落的剂量依赖性的方式，在两个采样时间观察到类似的模式。修改了多个操作分类单位和噬菌体类型：富集了先前在人类和动物研究中与瘦型相关的噬菌体类型拟杆菌和颤螺旋菌;并且以剂量依赖性方式在GTP处理组中耗尽了先前与结直肠癌表型相关的消化链球菌科。几种微生物基因的直系同源物进行了修改，其中与能源生产和转换的基因一直丰富的样品从第6个月的剂量依赖性的方式。这项研究表明，长期使用GTP治疗诱导了实验大鼠肠道微生物组的剂量依赖性改变，这可能与GTP的有益作用有关。
Green tea polyphenols (GTP) have been shown to exert a spectrum of health benefits to animals and humans. It is plausible that the beneficial effects of GTP are a result of its interaction with the gut microbiota. This study evaluated the effect of long-term treatment with GTP on the gut microbiota of experimental rats and the potential linkage between changes of the gut microbiota with the beneficial effects of GTP. Six-month-old Sprague-Dawley rats were randomly allocated into three dosing regimens (0, 0.5%, and 1.5% of GTP) and followed for 6 months. At the end of month 3 or month 6, half of the animals from each group were sacrificed and their colon contents were collected for microbiome analysis using 16S ribosomal RNA and shotgun metagenomic community sequencing. GTP treatment significantly decreased the biodiversity and modified the microbial community in a dose-dependent manner; similar patterns were observed at both sampling times. Multiple operational taxonomic units and phylotypes were modified: the phylotypes Bacteroidetes and Oscillospira, previously linked to the lean phenotype in human and animal studies, were enriched; and Peptostreptococcaceae previously linked to colorectal cancer phenotype was depleted in GTP treated groups in a dose-dependent manner. Several microbial gene orthologs were modified, among which genes related to energy production and conversion were consistently enriched in samples from month 6 in a dose-dependent manner. This study showed that long-term treatment with GTP induced a dose-dependent modification of the gut microbiome in experimental rats, which might be linked to beneficial effects of GTP.