THE APPEARANCE OF FREE HYDROXYPROLINE AS THE MAJOR PRODUCT OF DEGRADATION OF NEWLY SYNTHESIZED COLLAGEN IN CELL-CULTURE
THE APPEARANCE OF FREE HYDROXYPROLINE AS THE MAJOR PRODUCT OF DEGRADATION OF NEWLY SYNTHESIZED COLLAGEN IN CELL-CULTURE
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DOI：
10.1016/0304-4165(82)90236-7
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
FRANZBLAU, C
中科院分区：
文献类型：
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作者：
IMBERMAN, M;OPPENHEIM, F;FRANZBLAU, C
Embryonic lung fibroblasts and rabbit vascular smooth muscle cells have the ability to degrade newly synthesized collagen. Analysis of 24 h pulse media from cultures given [14C]proline demonstrates that > 90% of the degraded collagen is represented by free hydroxyproline rather than the peptide bound imino acid. The addition of cycloheximide or .alpha.-.alpha.-dipyridyl to the culture medium during the pulse period severely diminished the formation of the free hydroxyproline demonstrating its enzymatic and protein (collagen) origin. Assessment of free hydroxyproline formation may allow intracellular and extracellular collagen degradation to be distinguished.
