In Vivo Assessment of Muscle Contractility in Animal Studies.
In Vivo Assessment of Muscle Contractility in Animal Studies.
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DOI：
10.1007/978-1-4939-3810-0_20
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Lovering RM
中科院分区：
文献类型：
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作者：
Iyer SR;Valencia AP;Hernández-Ochoa EO;Lovering RM
对于肌肉损伤或肌肉疾病的患者，肌肉损伤的评估通常仅限于临床体征，例如压痛、力量、运动范围以及最近的影像学研究。动物模型提供了对组织学样本的完整访问，从而提供了损伤的“直接测量”。然而，即使不受限制地进行组织形态和生物化学测定，这些发现通常也不能解释肌肉功能的丧失。因此，肌肉整体健康最全面的衡量标准是评估其主要功能，即产生收缩力。大多数测试收缩力的动物模型仅限于移动脚踝的肌肉群，其优点和缺点取决于设备。在这里，我们描述了测量扭矩的体内方法，以产生可靠的肌肉损伤，并随着时间的推移跟踪同一动物的肌肉功能。我们还描述了测量腿部和大腿肌肉张力的体内方法。
In patients with muscle injury or muscle disease, assessment of muscle damage is typically limited to clinical signs, such as tenderness, strength, range of motion, and more recently, imaging studies. Animal models provide unmitigated access to histological samples, which provide a “direct measure” of damage. However, even with unconstrained access to tissue morphology and biochemistry assays, the findings typically do not account for loss of muscle function. Thus, the most comprehensive measure of the overall health of the muscle is assessment of its primary function, which is to produce contractile force. The majority of animal models testing contractile force have been limited to the muscle groups moving the ankle, with advantages and disadvantages depending on the equipment. Here, we describe in vivo methods to measure torque, to produce a reliable muscle injury, and to follow muscle function within the same animal over time. We also describe in vivo methods to measure tension in the leg and thigh muscles.