Murine bone marrow cell line producing colony-stimulating factor.
Murine bone marrow cell line producing colony-stimulating factor.
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鼠骨髓细胞系产生集落刺激因子。
DOI：
10.1073/pnas.78.11.6963
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发表时间：
1981
影响因子：
11.1
通讯作者：
Shadduck,RK
中科院分区：
文献类型：
--
作者：
Harigaya,K;Cronkite,EP;Miller,ME;Shadduck,RK
在这些研究中，从14周大的Dexter骨髓培养物的贴壁层中提取的细胞系（H-1）作为克隆和非克隆细胞系经过了21代。这些细胞系随着年龄的增长和融合而产生许多脂肪滴。当细胞融合时，未克隆的细胞系产生越来越多的集落刺激活性。与含有集落刺激因子（CSF）的L细胞培养液相比，来自非融合或融合脂肪细胞的饲养层或上清液刺激集落形成单位（CFU）培养物产生更多数量的集落。来自L细胞培养的含csf培养基的抗体中和了集落刺激活性，因此显示出与CFU培养中刺激集落产生的已知分子物种的免疫学相似性，该分子物种产生粒细胞或巨噬细胞群体，或两者兼而有之。
A cell line (H-1) derived from the adherent layer of a 14-wk-old Dexter bone marrow culture has been maintained as cloned and uncloned lines through 21 passages at the time of these studies. These cell lines develop many fat droplets as they age and become confluent. The uncloned line produces increasing amounts of colony-stimulating activity as the cells become confluent. Feeder layers or supernatants from the nonconfluent or confluent fat-laden cells stimulate the formation of greater numbers of colonies derived from cultures of colony-forming units (CFU) than does medium from L cell culture containing colony-stimulating factor (CSF). Antibody to the CSF-containing medium from L cell culture neutralizes the colony-stimulating activity, thus showing immunologic similarity to a known molecular species that stimulates colony production in a CFU culture that produces granulocyte or macrophage populations, or both.