DNA content, kinetic complexity, and the ploidy question in Candida albicans.
DNA content, kinetic complexity, and the ploidy question in Candida albicans.
复制标题
白色念珠菌的 DNA 含量、动力学复杂性和倍性问题。
DOI：
10.1128/mcb.2.7.853-862.1982
复制
发表时间：
1982
影响因子：
5.3
通讯作者：
Townes,TM
中科院分区：
文献类型：
--
作者：
Riggsby,WS;Torres-Bauza,LJ;Wills,JW;Townes,TM
白色念珠菌是一种对人类有致病性的二型真菌。这种真菌没有性周期的报道，早期的报道对典型的C。白念珠菌是单倍体或二倍体。先前对C. albicans变化了一个数量级。我们使用三种独立的方法来测量来自典型的C菌株的单拷贝DNA的动力学复杂性。白色念珠菌（菌株H317），以确定每个单倍体基因的DNA含量，我们得到的值为15和20 fg每个细胞通过使用S1核酸酶和羟基磷灰石测定，分别。DNA再结合动力学的光学测定，虽然本身不是决定性的，但在此范围内产生值。几个典型的菌株，包括菌株H317的DNA含量的化学测量，产生的值聚类约37 fg每个细胞的平均值。我们的结论是，这些菌株是二倍体。
Candida albicansis a dimorphic fungus that is pathogenic for humans. No sexual cycle has been reported for this fungus, and earlier reports have differed on whether typical strains ofC. albicansare haploid or diploid. Previous estimates of the DNA content ofC. albicansvaried by one order of magnitude. We used three independent methods to measure the kinetic complexity of the single-copy DNA from a typical strain ofC. albicans(strain H317) to determine the DNA content per haploid genote; we obtained values of 15 and 20 fg per cell by using S1 nuclease and hydroxyapatite assays, respectively. Optical assays for DNA reassociation kinetics, although not definitive in themselves, yielded values in this range. Chemical measurements of the DNA content of several typical strains, including strain H317, yielded values clustered about a mean of 37 fg per cell. We concluded that these strains are diploid.