Molecular epidemiology of rotavirus gastroenteritis in Japan during 2014-2015: Characterization of re-emerging G2P[4] after rotavirus vaccine introduction
Molecular epidemiology of rotavirus gastroenteritis in Japan during 2014-2015: Characterization of re-emerging G2P[4] after rotavirus vaccine introduction
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DOI：
10.1002/jmv.25067
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发表时间：
2018-06-01
影响因子：
12.7
通讯作者：
Ushijima, Hiroshi
中科院分区：
文献类型：
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作者：
Khandoker, Nusrat;Thongprachum, Aksara;Ushijima, Hiroshi
自2011年以来，轮状病毒疫苗已在日本上市。本研究旨在监测接种疫苗3年后A组轮状病毒（RVA）基因型的变化趋势。2014年7月至2015年6月共收集6个地区急性胃肠炎患儿粪便样本539份。其中RVA阳性178例（33.0%）。主要基因型为G1P[8](35.9%)，其次为G2P[4]（26.4%）、G9P[8]（21.3%）、G3P[8]（4.5%）和G3P[9]（4.5%）。G2P[4]的检出率在疫苗引入后很快提高。对代表性G2P[4]菌株的VP7和VP4基因序列分析发现，VP7和VP4基因聚集在谱系IV的IVa亚系中。值得注意的是，在本研究的代表性G2P[4]菌株中，VP4基因的抗原表位（Q114P）有一个氨基酸替换。然而，目前尚不清楚抗原表位的变化是由于疫苗接种的影响还是由于自然变异，因此需要进一步持续监测疫苗引入后轮状病毒的演变。
Rotavirus vaccines have been available in Japan since 2011. This study conducted to monitor the trend of group A rotavirus (RVA) genotypes 3 years after vaccine introduction. A total of, 539 fecal samples were collected from children with acute gastroenteritis in six regions during July 2014-June 2015. Among them, 178 samples (33.0%) were positive for RVA. The most predominant genotype was G1P[8] (35.9%) followed by G2P[4] (26.4%), G9P[8] (21.3%), G3P[8] (4.5%), and G3P[9] (4.5%). The detection rate of G2P[4] was increased soon after vaccine introduction. Sequence analyses of VP7 and VP4 genes of the representative G2P[4] strains were found to be clustered in sub-lineage IVa of lineage IV. It is noteworthy that one amino acid substitution in the antigenic epitope (Q114P) of VP4 gene was found in representative G2P[4] strains of the current study. However, it is unclear whether the change in antigenic epitope is due to the effect of vaccination or due to natural variation, warranting further continuous monitoring of rotavirus evolution after vaccine introduction.