Fluorescence-based active site probes for profiling deubiquitinating enzymes
Fluorescence-based active site probes for profiling deubiquitinating enzymes
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DOI：
10.1039/c2ob25258a
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Kessler, Benedikt M.
中科院分区：
文献类型：
--
作者：
McGouran, Joanna F.;Kramer, Holger B.;Kessler, Benedikt M.
已经开发并评估了包括荧光标签的新型基于泛素的活性位点探针。一种新的，可官能化的亲电阱被利用，允许后期多样化的探针。荧光染料的附着允许通过凝胶内荧光成像直接检测细胞提取物中的内源性去泛素化酶（DUB）活性。
Novel ubiquitin-based active site probes including a fluorescent tag have been developed and evaluated. A new, functionalizable electrophilic trap is utilized allowing for late stage diversification of the probe. Attachment of fluorescent dyes allowed direct detection of endogenous deubiquitinating enzyme (DUB) activities in cell extracts by in-gel fluorescence imaging.