Distinct expression patterns of mitochondrially localized YFP in neuronal subsets in the retina of three transgenic mouse lines.
Distinct expression patterns of mitochondrially localized YFP in neuronal subsets in the retina of three transgenic mouse lines.
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DOI：
10.1186/1756-0500-3-253
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发表时间：
2010-10-06
影响因子：
1.8
通讯作者：
Fuerst PG
中科院分区：
文献类型：
--
作者：
Burgess RW;Fuerst PG
允许特定神经元群体可视化的转基因标签已被证明是强大的研究工具。进一步开发这些资源以标记这些细胞内的其他细胞类型和特定细胞器将提供额外的实验机会。在三个转基因小鼠品系中的每一个中，确定视网膜中的视网膜定位的黄色荧光蛋白（YFP）的表达谱。每种细胞系，Mito-R、Mito-Y和Mito-Z，在不同的和可重复的视网膜神经元群体中表达YFP。在Mito-R系中，YFP在大多数或所有视网膜神经节细胞（RGC）和光感受器中表达，使得该系可用于研究疾病如青光眼和与线粒体转运到内节相关的光感受器变性中的轴突转运。在Mito-Y系中，YFP在背侧视网膜中的许多细胞类型中以及在视网膜的其余部分中的RGC的粗糙镶嵌群中表达，使得该系可用于研究视网膜镶嵌是如何组织的。在Mito-Z系中，YFP在RGC、无长突细胞、双极细胞和光感受器的子集中表达。将Mito-Z系插入X染色体上，导致携带单拷贝转基因的雌性小鼠中的X失活嵌合体。在女性半合子视网膜中，表达存在于不同的克隆柱中，使得该转基因系可用于分析视网膜神经元的克隆增殖和侧向迁移。在这项研究中，视网膜表达谱的三个转基因小鼠系，表达一个naturally本地化的YFP。这些细胞系将使研究人员能够分离和鉴定视网膜内的细胞类型，并研究视网膜线粒体运输和疾病。
Transgenic labels that allow the visualization of specific populations of neurons have proven to be powerful tools for research. Further developing such resources to label additional cell types and specific organelles within these cell will provide additional experimental opportunities. The retinal expression profile of a mitochondria-localized yellow fluorescent protein (YFP) in each of three transgenic mouse lines was determined. Each line, Mito-R, Mito-Y and Mito-Z, expresses YFP in distinct and reproducible populations of retinal neurons. In the Mito-R line, YFP is expressed in most or all retinal ganglion cells (RGCs) and photoreceptors making this line useful for studying axonal transport in diseases such as glaucoma and photoreceptor degeneration related to transport of mitochondria into the inner segments. In the Mito-Y line, YFP is expressed in many cell types in the dorsal retina and in a rough mosaic population of RGCs in the rest of the retina, making this line useful for study of how retinal mosaics are organized. In the Mito-Z line, YFP is expressed in a subset of RGCs, amacrine cells, bipolar cells and photoreceptors. The Mito-Z line is inserted on the X-Chromosome, resulting in X-inactivation mosaicism in female mice carrying a single copy of the transgene. In the female hemizygous retina, expression is present in distinct clonal columns, making this transgenic line useful for analysis of clonal proliferation and lateral migration of retinal neurons. The retinal expression profiles of three transgenic mouse lines that express a mitochondrially localized YFP were characterized in this study. These lines will allow researchers to isolate and identify cell types within the retina and to study retinal mitochondrial trafficking and disease.