Inhibition of intestinal transit by resuscitation-induced gut edema is reversed by L-NIL
Inhibition of intestinal transit by resuscitation-induced gut edema is reversed by L-NIL
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DOI：
10.1016/j.jss.2005.04.041
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发表时间：
2005-11-01
影响因子：
2.2
通讯作者：
Cox, CS
中科院分区：
文献类型：
--
作者：
Moore-Olufemi, SD;Xue, H;Cox, CS
背景复苏后肠道水肿和相关的肠道功能障碍是创伤性损伤和休克后常见且重要的临床问题。我们之前已经证明，没有缺血/再灌注损伤的肠道水肿会延迟肠道转运[1]。我们假设肠水肿增加诱导型一氧化氮合酶（iNOS）蛋白的表达，并且使用L-NIL的选择性iNOS抑制逆转与肠水肿相关的延迟的肠转运。在剖腹手术前1小时，用10 mg/kg体重的腹膜内L-NIL或盐水载体预处理大鼠，并进行80 ml/kg体重的0.9%盐水+上级肠系膜静脉压升高（水肿）或假手术（Sham）。放置十二指肠导管以允许注射荧光染料用于测量肠道传输。在6 h时，分离小肠并测量荧光染料的平均几何中心（MGC）以确定转运。取小肠组织行组织学检查，Western blotting法检测iNOS蛋白表达，MPO活性。使用湿干重量比测定组织水以评估肠道水肿。数据表示为平均值+/- SEM，n = 3-6，* = P
Background. Post-resuscitation gut edema and associated gut dysfunction is a common and significant clinical problem that occurs after traumatic injury and shock. We have shown previously that gut edema without ischemia/reperfusion injury delays intestinal transit [1]. We hypothesized that gut edema increases expression of inducible nitric oxide synthase (iNOS) protein, and that selective iNOS inhibition using L-NIL reverses the delayed intestinal transit associated with gut edema.Materials and methods. One hour prior to laparotomy, rats were pretreated with 10 mg/kg body weight of intraperitoneal L-NIL or saline vehicle and underwent 80 ml/kg body weight of 0.9% saline + superior mesenteric venous pressure elevation (Edema) or sham surgery (Sham). A duodenal catheter was placed to allow injection of a fluorescent dye for the measurement of intestinal transit. At 6 h, the small bowel was divided and the mean geometric center (MGC) of fluorescent dye was measured to determine transit. Ileum was harvested for histological assessment of mucosal injury, evaluation of iNOS protein expression by Western blotting, and MPO activity. Tissue water was determined using the wet-to-dry weight ratio to assess gut edema. Data are expressed as mean +/- SEM, n = 3-6 and * = P