Concentration of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation
Concentration of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation
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DOI：
10.1093/humrep/17.1.213
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发表时间：
2002-01-01
影响因子：
6.1
通讯作者：
Chaouat, G
中科院分区：
文献类型：
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作者：
Lédée-Bataille, N;Laprée-Delage, G;Chaouat, G
背景：有强有力的证据表明，局部分泌的细胞因子控制种植过程，并可能导致种植失败。分析子宫冲洗液，以确定其白血病抑制因子（LIF）和肿瘤坏死因子（TNF）的浓度。方法和结果：我们开始于连续两个周期的第26天冲洗33例不孕症患者的宫腔。LIF（酶联免疫吸附测定法）和TNF（生物测定法）的浓度在这些周期中显著相关（分别为r = 0.762，P = 0.0001和r = 0.822，P = 0.001），因此是可靠的。然后，在30例不孕患者的参考冲洗后，我们跟踪了他们第一个连续周期的卵巢刺激的结果，这是在IVF或宫腔内人工授精之前。共有10名患者妊娠。在妊娠的患者中，LIF的中位浓度为0 pg/ml（范围：0-177），TNF的中位浓度为0 U/ml（范围：0-6.17），而在未妊娠的患者中，LIF的中位浓度为203 pg/ml（范围：0-1620），TNF的中位浓度为2.14 U/ml（范围：0-16）。妊娠组LIF浓度显著低于对照组（P = 0.0013）。结论：在第26天子宫冲洗液中低浓度的LIF可预测随后的植入。使用这种程序应该增加IVF尝试成功怀孕的次数，并导致纠正治疗。
BACKGROUND: There is strong evidence that locally secreted cytokines control the implantation process and can cause implantation failure. Uterine flushing fluids were analysed to determine their concentrations of leukaemia inhibitory factor (LIF) and tumour necrosis factor (TNF). METHODS AND RESULTS: We began by flushing the uterine cavities of 33 infertile patients on day 26 of two consecutive cycles. The concentrations of LIF (by enzyme-linked immunosorbent assay) and TNF (by bioassay) were significantly correlated during these cycles (r = 0.762, P = 0.0001 and r = 0.822, P = 0.001 respectively) and hence reliable. Then, after a reference flushing of 30 infertile patients, we followed the outcome of their first consecutive cycle of ovarian stimulation, which preceded either IVF or intrauterine insemination. A total of 10 patients became pregnant. The median concentration of LIF was 0 pg/ml (range: 0-177) and of TNF was 0 U/ml (range: 0-6.17) among those who became pregnant, and 203 pg/ml (range: 0-1620) and 2.14 U/ml (range: 0-16) respectively among those who did not. The LIF concentration was significantly lower in the pregnant group (P = 0.0013). CONCLUSION: A low concentration of LIF in the uterine flushing fluid at day 26 was predictive of subsequent implantation. Use of this procedure should increase the number of IVF attempts yielding successful pregnancies and also lead to corrective therapies.