Targeting of Wolbachia endobacteria in Litomosoides sigmodontis:: comparison of tetracyclines with chloramphenicol, macrolides and ciprofloxacin
Targeting of Wolbachia endobacteria in Litomosoides sigmodontis:: comparison of tetracyclines with chloramphenicol, macrolides and ciprofloxacin
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DOI：
10.1046/j.1365-3156.2000.00544.x
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发表时间：
2000-04-01
影响因子：
3.3
通讯作者：
Fleischer, B
中科院分区：
文献类型：
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作者：
Hoerauf, A;Volkmann, L;Fleischer, B
丝虫线虫中沃尔巴克氏体属的内生细菌与立克次体科有关，可被四环素抗生素消灭。这种消耗会阻止雌性蠕虫发育以及早期胚胎发生，而目前使用的杀微丝虫伊维菌素仅阻止胚胎发生的最后阶段。由于针对沃尔巴克氏体正在成为治疗人类丝虫病的一个研究领域，因此研究了四环素以外的抗生素是否能够消除丝虫中的沃尔巴克氏体。用红霉素、氯霉素或环丙沙星治疗感染啮齿类丝虫乙状丝虫的 BALB/c 小鼠。所有药物都被很好地吸收，并且导致血清水平明显高于对相应抗生素敏感的细菌的断点水平。然而，与四环素相反，免疫组织学、免疫电子显微镜和半定量 PCR 证明，这些抗生素都不能消除沃尔巴克氏体或改变蠕虫的发育和繁殖力。
Endobacteria of the genus Wolbachia in filarial nematodes are related to Rickettsiaceae and can be depleted by tetracycline antibiotics. This depletion blocks female worm development as well as early embryogenesis, in contrast to the currently used microfilaricidal ivermectin which blocks only the last stage of embryogenesis. Since targeting Wolbachia is becoming an area of research for the treatment of human filariases, it was investigated if antibiotics other than tetracyclines are able to deplete Wolbachia from filariae. BALB/c mice infected with the rodent filaria Litomosoides sigmodontis were treated with erythromycin, chloramphenicol or ciprofloxacin. All drugs were well resorbed and resulted in serum levels clearly above breakpoint levels for bacteria susceptible to the respective antibiotic. However, contrary to tetracycline, none of these antibiotics depleted Wolbachia or altered worm development and fertility, as evidenced by immunohistology, immunoelectron microscopy and semiquantitative PCR.