The whole transcriptome and proteome changes in the early stage of myocardial infarction
The whole transcriptome and proteome changes in the early stage of myocardial infarction
复制标题
心肌梗死早期全转录组和蛋白质组变化
DOI：
10.1038/s41420-019-0152-z
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发表时间：
2019-03-04
影响因子：
7
通讯作者：
Shen, Junwei
中科院分区：
文献类型：
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作者：
Li, Yanfei;Wang, Cuiping;Shen, Junwei
心肌梗死（MI）是冠状动脉疾病最严重的表现，是一个复杂的多因素的病理生理过程。然而，心肌梗死的发病机制尚不清楚。在这里，我们通过结扎冠状动脉左前降支近端建立了MI小鼠模型。检测并分析心肌梗死后不同时间点的转录组和蛋白质组。MI后免疫相关通路、细胞周期相关通路和细胞外基质重塑相关通路显著增加。在MI的早期，不仅有先天性免疫细胞参与，而且有适应性免疫细胞参与。心肌梗死后血凝、纤溶、分泌、免疫等功能蛋白发生明显变化。Nppa、Serpina3n和Anxa1这三种在血液中容易检测到的分泌蛋白在MI后显著改变。我们的发现不仅揭示了MI的分子和细胞变化，而且还确定了MI的潜在候选生物标志物，用于临床诊断或治疗。
As the most severe manifestation of coronary artery disease, myocardial infarction (MI) is a complex and multifactorial pathophysiologic process. However, the pathogenesis that underlies MI remains unclear. Here, we generated a MI mouse model by ligation of the proximal left anterior descending coronary artery. The transcriptome and proteome, at different time points after MI, were detected and analysed. Immune-related pathways, cell cycle-related pathways, and extracellular matrix remodelling-related pathways were significantly increased after MI. Not only innate immune cells but also adaptive immune cells participated in the early stage of MI. Proteins that functioned in blood agglutination, fibrinolysis, secretion, and immunity were significantly changed after MI. Nppa, Serpina3n, and Anxa1, three secreted proteins that can easily be detected in blood, were significantly changed after MI. Our discoveries not only reveal the molecular and cellular changes in MI but also identify potential candidate biomarkers of MI for clinical diagnosis or treatment.