Studies on the control region of the p10 gene of the Autographa californica nuclear polyhedrosis virus.
Studies on the control region of the p10 gene of the Autographa californica nuclear polyhedrosis virus.
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苜蓿银纹夜蛾核型多角体病毒p10基因控制区的研究。
DOI：
10.1099/0022-1317-70-5-1273
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Weaver,RF
中科院分区：
文献类型：
--
作者：
Qin,JC;Liu,AF;Weaver,RF
已经制备了苜蓿银纹夜蛾核多角体病毒非常晚的p 10基因启动子的5'缺失突变体，并在受感染的草地贪夜蛾细胞中进行了瞬时表达测定。对照质粒含有受 p 10 启动子控制的氯霉素乙酰转移酶 (CAT) 报告基因，该基因包含在 p 10 翻译起始密码子上游的 230 bp 序列中。对照质粒还含有 CAT 基因下游的 hr5enhancer 片段。启动子活性不受 –77 位 5' 缺失的影响，该位置位于 p10 帽位点上游约 11 bp 处。然而，再删除 12 个 bp 就完全消除了 p10 启动子活性。因此，p10 启动子的 5' 边界位于 –77 位的下游，并且 –77 位和 –65 位之间的区域包含一个对启动子活性很重要的元件。这是晚期杆状病毒基因帽位点附近的保守区域。我们的研究还表明，当转染发生在病毒感染之前时，p10 启动子和 hr5 增强子控制下的 CAT 瞬时表达较高。
5′ deletion mutants of theAutographa californicanuclear polyhedrosis virus very late p 10 gene promoter have been prepared and subjected to a transient expression assay in infectedSpodoptera frugiperdacells. The control plasmid contained the chloramphenicol acetyltransferase (CAT) reporter gene under the control of the p 10 promoter, which was included in a 230 bp sequence upstream from the p 10 translation initiation codon. The control plasmid also contained a segment of the hr5enhancer downstream from the CAT gene. Promoter activity was unaffected by 5′ deletion to position –77, which lies about 11 bp upstream from the p10 cap site. However, deletion of 12 more bp completely eliminated p10 promoter activity. Thus, the 5′ border of the p10 promoter lies downstream from position –77, and the region between positions –77 and –65 contains an element that is important to promoter activity. This is the region that is conserved near the cap sites of late baculovirus genes. Our studies also show that transient expression of CAT under the control of the p10 promoter and hr5enhancer is higher when transfection occurs prior to infection by virus.