Efficient plant regeneration via organogenesis in winter squash (Cucurbita maxima Duch.)
Efficient plant regeneration via organogenesis in winter squash (Cucurbita maxima Duch.)
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DOI：
10.1016/s0168-9452(02)00429-6
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发表时间：
2003-03-01
期刊：
影响因子：
5.2
通讯作者：
Ezura, H
中科院分区：
文献类型：
--
作者：
Lee, YK;Chung, WI;Ezura, H
使用从体外萌发的幼苗上切下的子叶外植体，为两个冬南瓜（Cucurbita maxima Duch.）品种建立了通过器官发生的有效植物再生。为了建立不定芽诱导的最佳条件，从不同年龄的幼苗制备了多种外植体，并使用含有不同浓度的 6-苄氨基嘌呤 (BA) 的培养基培养这些外植体。对于两个品种。当 4 日龄幼苗的子叶近端部分在由 Murashige 和 Skoog (MS) 培养基（含 1 mg/l BA）组成的诱导培养基上培养时，植物再生效果最佳。在诱导培养基中培养 3 周后，两个品种的外植体分别有 82% 和 92% 再生出芽。将不定芽传代培养在含有0.1 mg/l BA的MS培养基组成的伸长培养基上，伸长的芽在不含生长调节剂的MS培养基中成功生根2周。流式细胞术分析表明大多数再生植物是二倍体。 (C) 2003 Elsevier Science Ireland Ltd. 保留所有权利。
Using cotyledonary explants excised from seedlings germinated in vitro, efficient plant regeneration via organogenesis was established for two winter squash (Cucurbita maxima Duch.) cultivars. To establish optimal conditions for adventitious shoot induction, a variety of explants were prepared from seedlings of different ages and these were cultured using media containing different concentrations of 6-benzylaminopurine (BA). For both cultivars. plant regeneration was optimal when the proximal parts of cotyledons from 4-day-old seedlings were cultured on induction medium composed of Murashige and Skoog (MS) medium with 1 mg/l BA. After 3 weeks of culture in induction medium, 82 and 92% of explants from the two cultivars regenerated shoots. Adventitious shoots were subcultured on elongation medium composed of MS medium with 0.1 mg/l BA and the elongated shoots were successfully rooted in MS medium without growth regulators for 2 weeks. Flow cytometric analysis revealed that most of the regenerated plants were diploid. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.