Rapid diagnosis of porcine epidemic diarrhea virus infection by polymerase chain reaction
Rapid diagnosis of porcine epidemic diarrhea virus infection by polymerase chain reaction
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DOI：
10.1292/jvms.59.231
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发表时间：
1997-03-01
影响因子：
1.2
通讯作者：
Kang, YB
中科院分区：
文献类型：
--
作者：
Kweon, CH;Lee, JG;Kang, YB
猪流行性腹泻病毒（PEDV）感染的实验室诊断由于病毒难以传播而相当繁琐。病毒在体内繁殖的可行性也受到同时处理多个样本的限制。本研究采用逆转录聚合酶链反应（RT-PCR）检测PEDV。RT-PCR可检出10(4)TCID50/ml的PEDV，与传染性胃肠炎病毒和猪轮状病毒无交叉反应。采用该方法，早在接种后1天就可以检测到PEDV。这些结果表明，RT-PCR可用于PEDV感染的快速诊断。
The diagnosis of porcine epidemic diarrhea virus (PEDV) infection in the laboratory is rather fastidious because of difficulties in virus propagation. The feasibility of virus propagation in vivo is also limited by the handling of a number of samples at the same time. In this study, the detection of PEDV by reverse transcription polymerase chain reaction (RT-PCR) is described. The RT-PCR could detect up to 10(4) TCID50/ml of PEDV and did not show any cross reaction with transmissible gastroenteritis virus or porcine rotavirus. Using this method, the detection of PEDV in experimentally inoculated piglets was possible as early as one day after inoculation. These results suggest that the RT-PCR could be applicable for a rapid diagnosis of PEDV infection.