Cryogenic spinal cord injury induces astrocytic gene expression of insulin‐like growth factor I and insulin‐like growth factor binding protein 2 during myelin regeneration
Cryogenic spinal cord injury induces astrocytic gene expression of insulin‐like growth factor I and insulin‐like growth factor binding protein 2 during myelin regeneration
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低温脊髓损伤在髓磷脂再生过程中诱导星形胶质细胞胰岛素样生长因子I和胰岛素样生长因子结合蛋白2的基因表达
DOI：
10.1002/jnr.490400510
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发表时间：
1995
影响因子：
4.2
通讯作者：
H. Webster
中科院分区：
文献类型：
--
作者：
D. Yao;N. R. West;C. Bondy;Michaela Brenner;L. Hudson;Jian Zhou;George H. Collins;H. Webster
为了研究损伤诱导的星形细胞反应与轴突再生和髓鞘再生相关，Collins及其同事采用了成年大鼠脊髓局灶低温损伤的方法（Collins et al.: J Neuropathol Exp Neurol 45:742-757, 1986）。低温损伤（CI）的持续时间，低温的大小和温度的选择，破坏所有髓鞘和轴突而不产生空腔或出血。采用原位杂交和免疫细胞化学方法，研究胰岛素样生长因子I （IGF‐I）、IGF‐I受体（IGFR‐I）、IGF结合蛋白2 （IGFBP‐2）、胶质纤维酸性蛋白（GFAP）和髓鞘碱性蛋白（MBP）在CI后3-56天的信使RNA和肽分布。在第3天，vimentin阳性，GFAP阴性的病变星形胶质细胞样细胞表达IGF - I mRNA和肽，CI后7天，两者都由典型的GFAP阳性，肥厚的星形胶质细胞表达，其中许多也是vimentin阳性。14天后，病变星形胶质细胞中IGF - 1、IGFBP - 2、GFAP mRNA和肽的水平升高。它们在第21-28天达到最高水平，然后在第56天下降到接近对照水平。CI后7-14天，病变内及周围少突胶质细胞MBP mRNA相对水平下降；随后，水平升高伴随着髓鞘再生。在CI后28和56天，一些转铁蛋白阳性的少突胶质细胞样细胞也被抗IGFR - I免疫染色。我们的研究结果表明，IGF - 1和IGFBP - 2的早期星形细胞生成可能参与了脊髓损伤模型中的髓鞘再生。©1995 Wiley‐Liss, Inc。这篇文章是美国政府的作品，因此，在美利坚合众国属于公有领域。
To study injury‐induced astrocytic responses associated with regrowth of axons and regeneration of myelin, the method of Collins and colleagues was used to make focal cryogenic lesions in spinal cords of adult rats (Collins et al.: J Neuropathol Exp Neurol 45:742–757, 1986). The duration of cryogenic injury (CI), the size of the cryode, and its temperature were chosen to destroy all myelin sheaths and axons without producing cavities or hemorrhages. Messenger RNA and peptide distributions of insulin‐like growth factor I (IGF‐I), IGF‐I receptor (IGFR‐I), IGF binding protein 2 (IGFBP‐2), glial fibrillary acidic protein (GFAP), and myelin basic protein (MBP) were studied 3–56 days after CI by in situ hybridization and immunocytochemistry. At 3 days, vimentin‐positive, GFAP‐negative astrocyte‐Iike cells in the lesion expressed IGF‐I mRNA and peptide and 7 days after CI, both were expressed by typical GFAP‐positive, hypertrophic astrocytes, many of which also were vimentin‐positive. Levels of IGF‐I, IGFBP‐2, and GFAP mRNA and peptide were higher in lesion astrocytes after 14 days. They attained maximum levels at 21–28 days before declining to near control levels at 56 days. Decreasing relative levels of oligodendroglial MBP mRNA were found in and around lesions 7–14 days after CI; subsequently, rising levels accompanied remyelination. At 28 and 56 days after CI, some transferrin‐positive, oligodendroglia‐like cells also were immunostained by anti‐IGFR‐I. Our findings suggest that early astrocytic production of IGF‐I and IGFBP‐2 may be involved in the myelin regeneration which occurs in this model of spinal cord injury. © 1995 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.
影响因子：
4.8
作者：
MATHEWS, LS;HAMMER, RE;PALMITER, RD
通讯作者：
PALMITER, RD