Gene expression in Porphyromonas gingivalis after contact with human epithelial cells
Gene expression in Porphyromonas gingivalis after contact with human epithelial cells
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DOI：
10.1128/iai.73.4.2327-2335.2005
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Duncan, MJ
中科院分区：
文献类型：
--
作者：
Hosogi, Y;Duncan, MJ
牙龈卟啉单胞菌是一种革兰氏阴性口腔厌氧菌，与成人牙周炎密切相关。当细菌开始感染和定植时，生物体与宿主上皮细胞的粘附发出两种细胞类型变化的信号，上皮细胞聚集它们的防御。我们假设在HEp-2人上皮细胞感染期间，一组确定的牙龈卟啉单胞菌基因的表达将持续上调。牙龈卟啉单胞菌基因组微阵列用于比较粘附于HEp-2细胞的细菌和单独孵育的细菌的基因表达谱。表达暂时上调的基因包括那些参与氧化应激反应的基因和那些编码热休克蛋白的基因，这些蛋白对于在不利条件下维持细胞活力至关重要。结果表明，与上皮细胞的接触诱导牙龈卟啉单胞菌的应激反应途径，促进细菌的存活。
Porphyromonas gingivalis, a gram-negative oral anaerobe, is strongly associated with adult periodontitis. The adherence of the organism to host epithelium signals changes in both cell types as bacteria initiate infection and colonization and epithelial cells rally their defenses. We hypothesized that the expression of a defined set of P. gingivalis genes would be consistently up-regulated during infection of HEp-2 human epithelial cells. P. gingivalis genome microarrays were used to compare the gene expression profiles of bacteria that adhered to HEp-2 cells and bacteria that were incubated alone. Genes whose expression was temporally up-regulated included those involved in the oxidative stress response and those encoding heat shock proteins that are essential to maintaining cell viability under adverse conditions. The results suggest that contact with epithelial cells induces in P. gingivalis stress-responsive pathways that promote the survival of the bacterium.