PROTEIN ORGANIZATION IN CLATHRIN TRIMERS
PROTEIN ORGANIZATION IN CLATHRIN TRIMERS
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DOI：
10.1016/0092-8674(81)90439-6
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
HARRISON, SC
中科院分区：
文献类型：
--
作者：
KIRCHHAUSEN, T;HARRISON, SC
从小牛脑包被囊泡制备均质可溶性8.6S物质（8.6S网格蛋白）。交联实验表明，该8.6S网格蛋白由3条重链（MW 180，000）和3条轻链（MW 33，000和36，000）组成。每条重链与单个轻链紧密接触，并且轻链似乎彼此不接触。完整的8.6S网格蛋白可以重新组装到笼中，而无需额外的蛋白质种类参与。
A homogeneous, soluble 8.6S species (8.6S clathrin) was prepared from calf-brain coated vesicles. Crosslinking experiments show that this 8.6S clathrin is composed of 3 heavy chains (MW 180,000) and 3 light chains (MW 33,000 and 36,000). Each heavy chain is in close contact with a single light chain, and the light chains appear not to be in contact with each other. Intact 8.6S clathrin can reassemble into cages without participation of additional protein species.