ENZYMATIC-PROPERTIES OF THE STONEFISH (SYNANCEIA-VERRUCOSA BLOCH AND SCHNEIDER, 1801) VENOM AND PURIFICATION OF A LETHAL, HYPOTENSIVE AND CYTOLYTIC FACTOR
ENZYMATIC-PROPERTIES OF THE STONEFISH (SYNANCEIA-VERRUCOSA BLOCH AND SCHNEIDER, 1801) VENOM AND PURIFICATION OF A LETHAL, HYPOTENSIVE AND CYTOLYTIC FACTOR
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DOI：
10.1016/0041-0101(94)00151-w
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发表时间：
1995-02-01
期刊：
影响因子：
2.8
通讯作者：
PERRIERE, C
中科院分区：
文献类型：
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作者：
GARNIER, P;GOUDEYPERRIERE, F;PERRIERE, C
石鱼（Synanceia verrucosa）的粗毒液具有多种酶特性（透明质酸酶、八种酯酶和十种氨肽酶）。通过 DEAE 和羟基磷灰石色谱法分离疣毒素，然后在 Superdex 200 HR 10/30 上进行 FPLC 凝胶过滤。发现它是一种带有梅尔的糖蛋白。重量为322,000+/-2000，包含四个亚基，2α(83,000)和2β(78,000)。疣毒毒素作为粗毒液，对小鼠来说是致命的，它会溶血洗涤过的兔子红细胞，并导致麻醉大鼠的动脉压下降。
The crude venom of the stonefish (Synanceia verrucosa) possesses numerous enzymatic properties (hyaluronidase, eight esterases and ten aminopeptidases). Verrucotoxin was isolated by DEAE and hydroxyapatite chromatography, followed by FPLC gel filtration on Superdex 200 HR 10/30. It was found to be a glycoprotein with a mel. wt of 322,000 +/- 2000, comprising four subunits, 2 alpha (83,000) and 2 beta (78,000). Verrucotoxin, as the crude venom, is lethal for mice, haemolyses washed rabbit erythrocytes and induces a fall in arterial pressure in anaesthetized rats.