Intracellular and extracellular tumor pH measurement in a series of patients with oral cancer
Intracellular and extracellular tumor pH measurement in a series of patients with oral cancer
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DOI：
10.1097/scs.0b013e3180de63eb
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发表时间：
2007-09-01
影响因子：
0.9
通讯作者：
Altieri, Fabio
中科院分区：
文献类型：
--
作者：
Becelli, Roberto;Renzi, Giancarlo;Altieri, Fabio
反向pH梯度是糖酵解上调导致的肿瘤表型的固有特征。这对肿瘤细胞的增殖、侵袭、转移、耐药性和凋亡至关重要。理解肿瘤中pH调节机制对治疗意义重大。这是一项针对头颈部肿瘤患者进行中性或微碱性pH/细胞内pH（pHi）/细胞外pH（pHe）测量的大型前瞻性研究的初步报告。在手术期间，从6名癌症患者获取了4个样本：2个来自肿瘤部位，2个来自对侧区域或肿瘤附近的正常区域。对正常组织和肿瘤组织内的pHe和pHi进行了测量和比较。我们的数据表明，人类自发性肿瘤显示出与先前在动物肿瘤模型和细胞系分析中观察到的类似的反向梯度。
Reversed pH gradient is an intrinsic feature of tumor phenotype resulting from an upregulation of glycolysis. This is crucial for tumor cell proliferation, invasion, metastasis, drug resistance, and apoptosis. Comprehension of mechanisms of pH regulation in tumors is of paramount importance for therapeutic implications. This is a preliminary report of a larger prospective study dedicated to the measurements of neutral or slightly alkaline pH/extracellular pH (pHi/pHe) in human patients affected by tumors of the head and neck. During surgery, four specimens were obtained from six patients with cancer: two from the tumor site and two from contralateral areas or sane areas near the tumor. pHe and pHi were measured and compared within normal and neoplastic tissues. Our data indicate that human spontaneous tumors show similar reversed gradients as observed in previous analysis on animal tumor models and cell lines.