Determination of plasma free fatty acids by high-performance liquid chromatography with electrochemical detection
Determination of plasma free fatty acids by high-performance liquid chromatography with electrochemical detection
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DOI：
10.1006/abio.2000.4682
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发表时间：
2000-08-15
影响因子：
2.9
通讯作者：
Kusu, F
中科院分区：
文献类型：
--
作者：
Kotani, A;Fuse, T;Kusu, F
采用高效液相色谱 - 电化学检测法测定对照血清和人血浆中的游离脂肪酸（FFAs）。在相对于饱和甘汞电极 -415 mV的检测电位下，棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸和花生四烯酸的峰高与50 - 1600 pmol范围内的酸量呈线性关系。本血浆游离脂肪酸测定方法仅需10 μL血浆样本。该方法简单，血液预处理时间短。通过该方法，以一男一女两名受试者为对象，监测进食前后血浆FFA随血糖水平的变化。进食后血浆FFA有可能迅速降低，血糖升高。因此，本方法显示出具有临床应用潜力。（C）2000学术出版社
Determination of free fatty acids (FFAs) in control serum and human plasma was carried out by highperformance liquid chromatography with electrochemical detection. Peak height for palmitic, palmitoleic, stearic, oleic, linoleic, and arachidonic acids at a detection potential of -415 mV vs a saturated calomel electrode showed a linear relation to acid amount in the range 50-1600 pmol. The present method for plasma free fatty acid determination required only 10 mu L of plasma sample. The method is simple and the time for blood pretreatment is short. Change in plasma FFA with blood glucose level was monitored before and after meal ingestion by this method, using one male and one female subject. It was possible to rapidly reduce plasma FFA and increase blood glucose subsequent to the meal. The present method is thus shown to have potential for clinical application. (C) 2000 Academic Press.