Cloning and sequencing of a gene encoding a new member of the tetratricopeptide protein family from magnetosomes of Magnetospirillum magnetotacticum
Cloning and sequencing of a gene encoding a new member of the tetratricopeptide protein family from magnetosomes of Magnetospirillum magnetotacticum
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DOI：
10.1016/0378-1119(95)00008-9
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发表时间：
1996-05-24
期刊：
影响因子：
3.5
通讯作者：
Fukumori, Y
中科院分区：
文献类型：
--
作者：
Okuda, Y;Denda, K;Fukumori, Y
从趋磁细菌Magnetotacellum magnetotacticum的磁小体中克隆了一个编码22 kDa蛋白（MAM22）的基因，并进行了序列测定。MAM22由220个氨基酸组成，分子量为24 186 Da，与线粒体蛋白输入受体和过氧化物酶体蛋白输入受体等TPR蛋白家族成员具有高度同源性。简并的34个氨基酸的共有序列的三个重复的存在下，表明MAM22定位在磁小体膜可能与含有类似TPR基序的细胞质蛋白相互作用。
A gene encoding the 22-kDa protein (MAM22) which was localized in the magnetosomes isolated from the magnetotactic bacterium, Magnetospirillum magnetotacticum, was cloned and sequenced. MAM22 was composed of 220 amino acids (aa) with a molecular weight of 24 186 Da. The deduced aa sequence exhibited significant homology with a number of proteins that belong to the tetratricopeptide repeat (TPR) protein family, including mitochondrial protein import receptors and peroxisomal protein import receptors. The presence of three repeats of a degenerate 34-aa consensus sequence, suggest that MAM22 localized in magnetosome membranes may interact with the cytoplasmic proteins containing similar TPR motifs.