Intake and time dependence of blueberry flavonoid-induced improvements in vascular function: a randomized, controlled, double-blind, crossover intervention study with mechanistic insights into biological activity
Intake and time dependence of blueberry flavonoid-induced improvements in vascular function: a randomized, controlled, double-blind, crossover intervention study with mechanistic insights into biological activity
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DOI：
10.3945/ajcn.113.066639
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发表时间：
2013-11-01
影响因子：
7.1
通讯作者：
Spencer, Jeremy P. E.
中科院分区：
文献类型：
--
作者：
Rodriguez-Mateos, Ana;Rendeiro, Catarina;Spencer, Jeremy P. E.
背景资料：关于蓝莓类黄酮摄入量对健康人血管功能的影响的数据非常有限。目的：我们研究了蓝莓类黄酮摄入量对健康男性内皮功能的影响，并通过评估循环代谢物和中性粒细胞NADPH氧化酶活性来评估潜在的作用机制。设计：对21名健康男性进行了两项随机、对照、双盲、交叉的人类干预试验。最初，蓝莓类黄酮摄入量对血流介导的扩张（FMD）和多酚吸收和代谢的影响进行了评估，在基线和1，2，4和6小时后，蓝莓含有766，1278和1791毫克总蓝莓多酚或常量营养素和微量营养素匹配的对照饮料（0毫克总蓝莓多酚）。第二，进行了摄入依赖性研究（从基线到1小时）与319，637，766，1278，和1791毫克总蓝莓多酚和control.Results：我们观察到一个双相的FMD的时间依赖性增加，在1-2和6小时后消费的蓝莓多酚显着增加。在766和1791 mg之间未观察到显著的摄入依赖性。然而，在食用后1小时，FMD呈剂量依赖性增加，
Background: There are very limited data regarding the effects of blueberry flavonoid intake on vascular function in healthy humans.Objectives: We investigated the impact of blueberry flavonoid intake on endothelial function in healthy men and assessed potential mechanisms of action by the assessment of circulating metabolites and neutrophil NADPH oxidase activity.Design: Two randomized, controlled, double-blind, crossover human-intervention trials were conducted with 21 healthy men. Initially, the impact of blueberry flavonoid intake on flow-mediated dilation (FMD) and polyphenol absorption and metabolism was assessed at baseline and 1, 2, 4, and 6 h after consumption of blueberry containing 766, 1278, and 1791 mg total blueberry polyphenols or a macronutrientand micronutrient-matched control drink (0 mg total blueberry polyphenols). Second, an intake-dependence study was conducted (from baseline to 1 h) with 319, 637, 766, 1278, and 1791 mg total blueberry polyphenols and a control.Results: We observed a biphasic time-dependent increase in FMD, with significant increases at 1-2 and 6 h after consumption of blueberry polyphenols. No significant intake-dependence was observed between 766 and 1791 mg. However, at 1 h after consumption, FMD increased dose dependently to