Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster.
Fasting, but Not Aging, Dramatically Alters the Redox Status of Cysteine Residues on Proteins in Drosophila melanogaster.
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DOI：
10.1016/j.celrep.2015.10.048
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发表时间：
2015-11-10
期刊：
影响因子：
8.8
通讯作者：
Murphy MP
中科院分区：
文献类型：
--
作者：
Menger KE;James AM;Cochemé HM;Harbour ME;Chouchani ET;Ding S;Fearnley IM;Partridge L;Murphy MP
改变蛋白质表面半胱氨酸残基的氧化还原状态是应对环境挑战的重要反应。尽管衰老和禁食会改变许多氧化还原过程，但半胱氨酸残基的作用尚不确定。为了解决这个问题，我们使用氧化还原蛋白质组学技术，即氧化同位素编码亲和标签（OxICAT）来评估果蝇在衰老和禁食期间的半胱氨酸残基氧化还原变化。这种方法使我们能够同时识别和量化体内数百个半胱氨酸残基的氧化还原状态。幼果蝇中的半胱氨酸残基呈双峰分布，峰值位于~10%和~85%可逆氧化。令人惊讶的是，这些半胱氨酸残基并没有随着年龄的增长而变得更加氧化。相比之下，禁食 24 小时会显着氧化半胱氨酸残基，而这些残基在进食条件下会减少，同时也会减少最初被氧化的半胱氨酸残基。我们得出的结论是，禁食（而非衰老）显着改变了 D 中的半胱氨酸残基氧化还原状态。 黑腹果蝇。
Altering the redox state of cysteine residues on protein surfaces is an important response to environmental challenges. Although aging and fasting alter many redox processes, the role of cysteine residues is uncertain. To address this, we used a redox proteomic technique, oxidative isotope-coded affinity tags (OxICAT), to assess cysteine-residue redox changes inDrosophila melanogasterduring aging and fasting. This approach enabled us to simultaneously identify and quantify the redox state of several hundred cysteine residues in vivo. Cysteine residues within young flies had a bimodal distribution with peaks at ∼10% and ∼85% reversibly oxidized. Surprisingly, these cysteine residues did not become more oxidized with age. In contrast, 24 hr of fasting dramatically oxidized cysteine residues that were reduced under fed conditions while also reducing cysteine residues that were initially oxidized. We conclude that fasting, but not aging, dramatically alters cysteine-residue redox status inD. melanogaster.
影响因子：
8.8
作者：
Menger KE;James AM;Cochemé HM;Harbour ME;Chouchani ET;Ding S;Fearnley IM;Partridge L;Murphy MP
通讯作者：
Murphy MP