A PLASMID ENCODING ENZYMES FOR NYLON OLIGOMER DEGRADATION - NUCLEOTIDE-SEQUENCE AND ANALYSIS OF POAD2
A PLASMID ENCODING ENZYMES FOR NYLON OLIGOMER DEGRADATION - NUCLEOTIDE-SEQUENCE AND ANALYSIS OF POAD2
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DOI：
10.1099/13500872-141-10-2585
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发表时间：
1995-10-01
期刊：
影响因子：
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通讯作者：
URABE, I
中科院分区：
文献类型：
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作者：
KATO, K;OHTSUKI, K;URABE, I
测定了黄杆菌（Flavobacterium sp. KI 723 T1）尼龙低聚物降解质粒pOAD 2的全序列。pOAD 2包含45519 bp，具有66.6 mol% G+C含量。在该质粒上鉴定了四种尼龙低聚物降解基因，即nylA（6-氨基己酸-环-二聚体水解酶基因）、nylB（6-氨基己酸-二聚体水解酶）、nylB'（与nylB具有88%同源性的基因）和nylC（内切型6-氨基己酸低聚物水解酶）的精确基因座，以及五个IS 6100元件。与GenBank和EMBL数据库比较，pOAD 2的8个氨基酸序列与oppA-F（oligopeptide permeases）、ftsX（deletion temperature sensitive）、pendE（isopenicillin N-acyltransferase）和rep（plasmid incompatibility）等基因产物的序列有很高的相似性。给出了pOAD 2的功能图。
The entire nucleotide sequence of nylon oligomer degradative plasmid pOAD2 from Flavobacterium sp. KI723T1 was determined. pOAD2 comprises 45519 bp, with a 66.6 mol% G+C content. The precise loci of the four nylon oligomer degradation genes, namely nylA (6-aminohexanoate-cyclic-dimer hydrolase gene), nylB (6-aminohexanoate-dimer hydrolase), nylB' (a gene having 88% homology to nylB) and nylC (endo-type 6-aminohexanoate oligomer hydrolase), and five IS6100 elements were identified on this plasmid. Comparison of the sequence of pOAD2 with those in the GenBank and EMBL databases revealed that the deduced amino acid sequences from eight regions of pOAD2 had significant similarity with the sequences of gene products such as oppA-F (oligopeptide permeases), ftsX (filamentation temperature sensitive), penDE (isopenicillin N-acyltransferase) and rep (plasmid incompatibility). A functional map of pOAD2 is presented.