STRUCTURE OF THE BOVINE TAU-GENE - ALTERNATIVELY SPLICED TRANSCRIPTS GENERATE A PROTEIN FAMILY
STRUCTURE OF THE BOVINE TAU-GENE - ALTERNATIVELY SPLICED TRANSCRIPTS GENERATE A PROTEIN FAMILY
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DOI：
10.1128/mcb.9.4.1389
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发表时间：
1989-04-01
影响因子：
5.3
通讯作者：
HIMMLER, A
中科院分区：
文献类型：
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作者：
HIMMLER, A
Tau是微管相关蛋白的主要类别，由分子量不均一的蛋白质家族组成。在先前描述的小鼠和牛tau蛋白的cDNA克隆中存在内部缺失，这表明转录本的选择性剪接可以解释蛋白质大小的异质性。牛tau基因的外显子-内含子结构的分析提供了通过体外扩增技术检测tau转录物的新变体所必需的序列信息。发现的变体转录物对应于缺失一个或多个外显子的mRNA种类，这表明通过在mRNA剪接期间跳过各种外显子，产生了一个蛋白质家族。通过与cDNA构建体的翻译产物和使用针对合成肽产生的抗血清进行比较，鉴定了从牛脑中分离的四种主要tau蛋白亚型。这些研究为分析阿尔茨海默病患者大脑中tau蛋白的潜在改变形式提供了试剂和基础。
Tau, a major class of microtubule-associated proteins, consists of a family of proteins that are heterogeneous in molecular weight. The presence of internal deletions in previously described cDNA clones for murine and bovine tau suggested that alternative splicing of transcripts could account for the protein size heterogeneity. Analysis of the exon-intron structure of the bovine tau gene provided sequence information necessary to detect new variants of tau transcripts by in vitro amplification techniques. The variant transcripts found corresponded to mRNA species missing one or more exons, which suggested that by skipping various exons during mRNA splicing, a family of proteins is generated. Four major tau protein isoforms isolated from bovine brain were identified by comparison with translation products of cDNA constructs and the use of antisera raised against synthetic peptides. These studies provide reagents and a basis for analyzing potentially altered forms of tau proteins in brains of patients with Alzheimer''s disease.