Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH
Conserved synteny of genes between chromosome 15 of Bombyx mori and a chromosome of Manduca sexta shown by five-color BAC-FISH
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DOI：
10.1139/g07-082
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发表时间：
2007-11-01
期刊：
影响因子：
3.1
通讯作者：
Yasukochi, Yuji
中科院分区：
文献类型：
--
作者：
Sahara, Ken;Yoshido, Atsuo;Yasukochi, Yuji
家蚕遗传连锁群的成功定位和大量细菌人工染色体（BAC）重叠群构建的第二代连锁图谱，以及公开的核苷酸序列数据库，为B染色体组型间的同线性研究提供了有力的工具。桑和其他鳞翅目物种。利用荧光原位杂交（BAC-FISH）技术，通过比较定位相应连锁群的正向同源基因，可以鉴定特定染色体间基因的保守同线性。这项技术是在B中建立的。mori的2个不同标记的BAC探针同时与粗线期二价体杂交。为了在鳞翅目昆虫中实现更高通量的BAC-FISH比较定位，我们开发了一种五色BAC-FISH方案，该方案允许我们同时将6种不同的BAC探针定位到B中的15号染色体。森。我们鉴定了6 B的直系同源物。mori LG 15基因（RpP 0、RpS 8、eIF 3、RpL 7A、RpS 23和Hsc 70）的BAC克隆，并从烟草天蛾（Manducasexta）中筛选出含有直系同源物的BAC克隆。Sexta BAC图书馆。所有6个M。sexta BAC克隆与单个M.粗线期精母细胞六价体。从而证实了B之间的保守同线性。mori第15号染色体和相应的M. Sexta染色体（因此暂时称为染色体15）。
The successful assignment of the existing genetic linkage groups (LGs) to individual chromosomes and the second-generation linkage map obtained by mapping a large number of bacterial artificial chromosome (BAC) contigs in the silkworm, Bombyx mori, together with public nucleotide sequence databases, offer a powerful tool for the study of synteny between karyotypes of B. mori and other lepidopteran species. Conserved synteny of genes between particular chromosomes can be identified by comparatively mapping orthologous genes of the corresponding linkage groups with the help of BAC-FISH (fluorescent in situ hybridization). This technique was established in B. mori for 2 differently labeled BAC probes simultaneously hybridized to pachytene bivalents. To achieve higher-throughput comparative mapping using BAC-FISH in Lepidoptera, we developed a protocol for five-color BAC-FISH, which allowed us to map simultaneously 6 different BAC probes to chromosome 15 in B. mori. We identified orthologs of 6 B. mori LG15 genes (RpP0, RpS8, eIF3, RpL7A, RpS23, and Hsc70) for the tobacco hornworm, Manduca sexta, and selected the ortholog-containing BAC clones from an M. sexta BAC library. All 6 M. sexta BAC clones hybridized to a single M. sexta bivalent in pachytene spermatocytes. Thus, we have confirmed the conserved synteny between the B. mori chromosome 15 and the corresponding M. sexta chromosome (hence provisionally termed chromosome 15).