Microbial diversity in saliva of oral squamous cell carcinoma.
Microbial diversity in saliva of oral squamous cell carcinoma.
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DOI：
10.1111/j.1574-695x.2010.00773.x
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发表时间：
2011-04
影响因子：
--
通讯作者：
Saxena D
中科院分区：
文献类型：
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作者：
Pushalkar S;Mane SP;Ji X;Li Y;Evans C;Crasta OR;Morse D;Meagher R;Singh A;Saxena D
口腔鳞状细胞癌（OSCC）的各个阶段都存在慢性炎症。这种炎症可能是由微生物持续的粘膜或上皮细胞定植引起的。越来越多的证据表明口腔细菌参与炎症，并需要进一步研究细菌在OSCC进展中的相关性。本研究的目的是评估口腔鳞状细胞癌患者唾液中细菌的多样性和相对丰度。使用454个平行DNA测序，对来自5名受试者的跨越核糖体RNA的V4-V5高变区的约58，000个PCR扩增子进行测序。共检出细菌8门（部）。大部分的分类序列属于门，厚壁菌门（45%）和拟杆菌门（25%）。此外，共鉴定出52个不同的属，约860个（16.51%）已知种，1077个（67%）序列属于各种未培养的细菌或未分类的组。基于丰度的覆盖率估计（ACE）和Chao 1获得的物种多样性估计值大于已发表的口腔其他微生物概况的分析。在所有三个OSCC受试者中存在15种独特的肿瘤类型。
In oral cavity chronic inflammation has been observed at various stages of oral squamous cell carcinomas (OSCC). This inflammation could result from persistent mucosal or epithelial cell colonization by microorganisms. There is an increasing evidence of the involvement of oral bacteria in inflammation and warrant further studies on the association of bacteria in the progression of OSCC. The objective of this study was to evaluate the diversity and relative abundance of bacteria in the saliva of subjects with OSCC. Using 454 parallel DNA sequencing, ~58,000 PCR amplicons that span the V4-V5 hypervariable region of ribosomal RNAs from 5 subjects were sequenced. Members of 8 phyla (divisions) of bacteria were detected. The majority of classified sequences belonged to phyla, Firmicutes (45%) and Bacteroidetes (25%). Further, a total of 52 different genera containing approximately 860 (16.51%) known species were identified, 1077 (67%) sequences belonged to various uncultured bacteria or unclassified group. The species diversity estimates obtained with abundance-based coverage estimators (ACE) and Chao1 were greater than published analyses of other microbial profiles from the oral cavity. Fifteen unique phylotypes were present in all three OSCC subjects.