Cytochalasin B and D dose-outflow facility response relationships in the cynomolgus monkey.
Cytochalasin B and D dose-outflow facility response relationships in the cynomolgus monkey.
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DOI：
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发表时间：
1982-11
影响因子：
4.4
通讯作者：
P. Kaufman;K. Erickson
中科院分区：
文献类型：
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作者：
P. Kaufman;K. Erickson
总流出设施通过在前房内输注细胞松弛素 B 和 D (CB、CD) 之前和之后对食蟹猴进行 30 至 40 分钟的两级恒压灌注来确定。定义了 CB 和 CD 剂量-设施反应关系。 0.1、0.2 和 0.5 微克的 CB 剂量为阈下剂量； 1、2、5 和 10 微克是有效的，其中 5 微克可能是超最大剂量。 0.02 微克的 CD 可能低于阈值； 0.1、0.5、1、2.5和5微克剂量是有效的，其中0.1微克接近或高于最大剂量。尽管两种药物的最大效果在数量上相似，大约是双倍启动能力，但 CD 的效力大约高出 25 倍。因此，CB 和 CD 诱导的功能增加可能主要是由于干扰小梁网内皮细胞和施莱姆氏管内壁肌动蛋白丝的形成、结构和功能。
Total outflow facility was determined by two-level constant-pressure perfusion in cynomolgus monkeys for 30 to 40 min before and after intracameral infusion of cytochalasins B and D (CB, CD). CB and CD dose-facility response relationships were defined. CB doses of 0.1, 0.2, and 0.5 micrograms were subthreshold; 1, 2, 5 and 10 micrograms were effective, with 5 micrograms probably supramaximal. CD at 0.02 microgram was probably subthreshold; 0.1, 0.5, 1, 2.5, and 5 micrograms doses were effective, with 0.1 microgram being near or above maximum. Although the maximum effects of both agents were quantitatively similar, approximately doubling-starting facility, CD Was about 25 times more potent. Therefore, CB- and CD-induced facility increases are presumably due largely to interference with formation, structure, and function of actin filaments in the endothelial cells of the trabecular meshwork and inner wall of Schlemm's canal.