MOLECULAR-CLONING AND SEQUENCING OF THE FIMBRILIN GENE OF PORPHYROMONAS-GINGIVALIS STRAINS AND CHARACTERIZATION OF RECOMBINANT PROTEINS
MOLECULAR-CLONING AND SEQUENCING OF THE FIMBRILIN GENE OF PORPHYROMONAS-GINGIVALIS STRAINS AND CHARACTERIZATION OF RECOMBINANT PROTEINS
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DOI：
10.1006/bbrc.1993.2467
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发表时间：
1993-11-30
影响因子：
3.1
通讯作者：
HAMADA, S
中科院分区：
文献类型：
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作者：
FUJIWARA, T;MORISHIMA, S;HAMADA, S
用聚合酶链反应（PCR）技术从9株牙龈卟啉单胞菌中克隆了编码菌毛亚单位蛋白fimbrilin的fimA基因，并测定了其核苷酸序列。核苷酸序列和推导的氨基酸序列分析表明，试验菌株的fimA基因由1044 ~ 1083 bp组成，推导的多肽分子量为37，527 ~ 38，239。所有供试菌株具有相同或相似的序列，但同时发现菌株之间的序列存在相当大的差异。使用兔抗血清toP进行蛋白质印迹分析。gingivalis 381菌毛中的8株重组菌毛蛋白在大肠杆菌中表达，与抗血清反应，显示43 ~ 48 kDa的条带。这些结果表明，在P.牙龈拉伤。
ThefimAgene encoding the subunit protein of fimbriae, fimbrilin, from nine strains ofPorphyromonas gingivaliswas cloned using polymerase chain reaction and the nucleotide sequence was determined. Analysis of the nucleotide and the deduced amino acid sequences revealed that thefimAgene of the test strains was composed of 1044 to 1083 bp, and the molecular weight of the deduced polypeptides was calculated to be 37,527 to 38,239. All the test strains shared the same or similar sequences, but simultaneously considerable differences in the sequences were found among the strains. Western blot analysis using rabbit antiserum toP. gingivalis381 fimbriae demonstrated that the recombinant fimbrilins of 8 out of 9 strains expressed inEscherichia colireacted with the antiserum exhibiting a 43 to 48 kDa band. Taken together, these results indicate that four genetical clusters are noted among thefimAgene ofP. gingivalisstrains.