Electrochemical desorption of self-assembled monolayers for engineering cellular tissues
Electrochemical desorption of self-assembled monolayers for engineering cellular tissues
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DOI：
10.1016/j.biomaterials.2009.03.045
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发表时间：
2009-07-01
期刊：
影响因子：
14
通讯作者：
Fukuda, Junji
中科院分区：
文献类型：
--
作者：
Inaba, Rina;Khademhosseini, Ali;Fukuda, Junji
粘附细胞，细胞片，和球状体收获非侵入性从培养表面的电化学解吸的自组装单层（SAM）的烷乙醇。SAM表面通过Arg-Gly-Asp（RGD）-肽与烷硫醇分子的共价键合而具有粘附性。施加负电位引起SAM的还原解吸，导致细胞的脱离。使用这种方法，超过90%的贴壁细胞在5分钟内脱落。此外，这种方法被用来获得二维（2D）的细胞片。分离的细胞片由活细胞组成，其可以容易地连续附着到其他细胞片以形成多层细胞片。此外，以280个球状体/cm（2）的密度在圆柱形空腔阵列中形成直径均匀的肝细胞球状体，并通过施加负电位收获。这种细胞操作技术可能是一个有用的工具，用于制造和组装的积木，如细胞片和球体再生医学和组织工程应用。(C)2009爱思唯尔有限公司保留所有权利。
Adherent cells, cell sheets, and spheroids were harvested noninvasively from a culture surface by means of electrochemical desorption of a self-assembled monolayer (SAM) of alkanethiol. The SAM surface was made adhesive by the covalent bonding of Arg-Gly-Asp (RGD)-peptides to the alkanethiol molecules. The application of a negative electrical potential caused the reductive desorption of the SAM, resulting in the detachment of the cells. Using this approach greater than 90% of adherent cells detached within 5 min. Furthermore, this approach was used to obtain two-dimensional (2D) cell sheets. The detached cell sheets consisted of viable cells, which could be easily attached to other cell sheets in succession to form a multilayered cell sheet. Moreover, spheroids of hepatocytes of a uniform diameter were formed in an array of cylindrical cavities at a density of 280 spheroids/cm(2) and were harvested by applying a negative electrical potential. This cell manipulation technology could potentially be a useful tool for the fabrication and assembly of building blocks such as cell sheets and spheroids for regenerative medicine and tissue engineering applications. (C) 2009 Elsevier Ltd. All rights reserved.