Efficient biodegradation of low-density polyethylene by cyanobacteria isolated from submerged polyethylene surface in domestic sewage water
Efficient biodegradation of low-density polyethylene by cyanobacteria isolated from submerged polyethylene surface in domestic sewage water
复制标题
从生活污水中浸没的聚乙烯表面分离出的蓝藻对低密度聚乙烯进行高效生物降解
DOI：
10.1007/s11356-018-3079-7
复制
发表时间：
2018
影响因子：
5.8
通讯作者：
J. Rout
中科院分区：
文献类型：
--
作者：
P. Sarmah;J. Rout
从生活污水中的聚乙烯手提袋中分离出两种优势蓝藻，分别是紫紫紫藻和紫紫紫藻，它们能够有效地降解低密度聚乙烯（LDPE）片。通过红外光谱（FT-IR）、扫描电镜（SEM）、核磁共振（NMR）、CHN含量、热强度和拉伸强度等检测PE的结构、形态和化学变化。CHN分析证实了来自PE的蓝藻物种的碳利用率约为4%。蓝藻种类在PE表面的快速生长表明微生物继续从PE获得能量。蓝藻处理过的聚乙烯板层厚度、重量和结晶度的减少表明，在没有任何促氧化剂添加剂或预处理的情况下，这是一种有效的生物降解过程。从FT-IR光谱计算的键指数的变化揭示了官能团和侧链特征的变化，表明生物降解。漆酶和锰过氧化物酶活性的增强证实了生物降解。聚乙烯的13c - nmr谱与短分支一致，为生物降解提供了进一步的证据。扫描电镜和光学显微镜显示，聚乙烯表面有较大的凹槽，表明聚乙烯结构明显破坏。
Two dominant cyanobacterial species,Phormidium lucidumandOscillatoria subbrevis, isolated from submerged polyethylene carry bags in domestic sewage water were found to be capable of degrading low-density polyethylene (LDPE) sheets efficiently. The FT-IR, SEM, NMR, CHN content, thermal, and tensile strength of PE were monitored for structural, morphological, and chemical changes of PE. The CHN analysis corroborated about 4% carbon utilization by the cyanobacterial species from the PE. The rapid growth of cyanobacterial species on the PE surface suggested that the microorganisms continued to gain energy from the PE. The reduction in lamellar thickness, weight, and crystallinity of the cyanobacterial-treated PE pointed to an efficient biodegradation process without any pro-oxidant additives or pretreatment. Alteration in bond indices computed from FT-IR spectroscopy revealed changes in functional group and side chain features indicating biodegradation. The enhanced laccase and manganese peroxidase activity corroborated the biodegradation. The13C-NMR spectroscopy of the PE is consistent with short branching providing further evidence of biodegradation. Scanning electron microscopy and optical microscopy exhibited large grooves on the surface suggesting significant disruption of polyethylene structure.