Quantifying serum antiplague antibody with a fiber-optic biosensor
Quantifying serum antiplague antibody with a fiber-optic biosensor
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DOI：
10.1128/cdli.5.5.609-612.1998
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发表时间：
1998-09-01
期刊：
影响因子：
--
通讯作者：
Ezzell, J
中科院分区：
文献类型：
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作者：
Anderson, GP;King, KD;Ezzell, J
光纤生物传感器，最初开发用于检测有害的生物制剂，如蛋白质毒素或细菌细胞，已被用于定量血清抗鼠疫抗体的浓度。该生物传感器已用于检测和定量血清、血浆和全血样本中的鼠疫组分I抗原，但其定量血清抗体的能力尚未得到证实。通过使用竞争性测定，确定血清抗鼠疫抗体的浓度在2至15 μ g/ml的范围内。通过简单的稀释，对11份血清样品的抗鼠疫抗体浓度未知的浓度进行了测定，发现与酶联免疫吸附试验结果吻合良好。竞争性检测方法可用于有效地确定动物或人类是否接触鼠疫，也可应用于其他疾病，如肝炎或艾滋病，其中抗体的存在可用于诊断感染。
The fiber-optic biosensor, originally developed to detect hazardous biological agents such as protein toxins or bacterial cells, has been utilized to quantify the concentration of serum antiplague antibodies. This biosensor has been used to detect and quantify the plague fraction I antigen in serum, plasma, and whole-blood samples, but its ability to quantify serum antibodies has not been demonstrated. By using a competitive assay, the concentration of serum antiplague antibodies was ascertained in the range of 2 to 15 mu g/ml. By making simple dilutions, concentrations for 11 serum samples whose antiplague antibody concentrations were unknown were determined and were found to be in good agreement with enzyme-linked immunosorbent assay results. The competitive assay method could be used to effectively determine the exposure to plague of animals or humans or could be applied to other diseases, such as hepatitis or AIDS, where the presence of antibodies is used to diagnose infection.