GROWTH WITH HYDROGEN, AND FURTHER PHYSIOLOGICAL-CHARACTERISTICS OF DESULFOTOMACULUM SPECIES
GROWTH WITH HYDROGEN, AND FURTHER PHYSIOLOGICAL-CHARACTERISTICS OF DESULFOTOMACULUM SPECIES
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DOI：
10.1007/bf00411048
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发表时间：
1985-01-01
影响因子：
2.8
通讯作者：
PFENNIG, N
中科院分区：
文献类型：
--
作者：
KLEMPS, R;CYPIONKA, H;PFENNIG, N
使用确定的矿物培养基研究了用硫酸盐和脂肪酸新分离的 Desulfotomaculum orientis、D. ruminis、D. nigrificans 和 Desulfotomaculum 菌株 TEP、TWC 和 TWP 的生长。其中四种菌株以氢加硫酸盐作为唯一能源生长。在这些条件下，分批培养中东方螟的生长产量为每摩尔硫酸减少7.5克细胞干质量。东方螟和菌株TEP在甲醇上生长，生长产量为每摩尔硫酸盐约6克细胞干质量。所有测试的菌株都以甲酸作为电子供体缓慢生长。 TEP、TWC 和 TWP 菌株利用从丙酸到棕榈酸的脂肪酸。 D. orientis 以及菌株 TEP 和 TWC 能够利用三甲氧基苯甲酸酯的甲氧基进行生长。人们发现东方东方蠹在氢气、二氧化碳和硫酸盐的作用下进行化能自养生长；在使用 C1 化合物生长期间，不需要额外的有机碳源。此外，东方东方螟能够在含有甲酸盐、甲醇、乳酸乙醇、丙酮酸盐或三甲氧基苯甲酸盐的无硫酸盐培养基中缓慢生长。在这些条件下，乙酸盐被排出，表明二氧化碳在同型乙酸过程中作为电子受体的功能。未观察到添加到 Desulfotomaculum 物种培养基中的焦磷酸盐的生长促进作用。结果表明，Desulfotomaculum 物种之间具有较高的分解代谢和合成代谢多功能性，并表明电子传输到硫酸盐可能是该属中唯一的能量守恒过程。
Growth of Desulfotomaculum orientis, D. ruminis, D. nigrificans and the Desulfotomaculum strains TEP, TWC and TWP, that were newly isolated with sulfate and fatty acids, was studied using defined mineral media. Four of these strains grew with hydrogen plus sulfate as the only energy source. Under these conditions the growth yield of D. orientis in batch culture was 7.5 g cell dry mass per mol sulfate reduced. Growth on methanol with growth yields of about 6 g cell dry mass per mol sulfate was obtained with D. orientis and strain TEP. All strains tested grew slowly with formate as electron donor. Fatty acids from propionate to palmitate were utilized by the strains TEP, TWC and TWP. D. orientis and the strains TEP and TWC were able to utilize the methoxyl groups of trimethoxybenzoate for growth. D. orientis was found to grow chemoautotrophically with hydrogen, carbon dioxide and sulfate; during growth with C1-compounds no additional organic carbon source was required. Furthermore, D. orientis was able to grow slowly in sulfate-free medium with formate, methanol, ethanol lactate, pyruvate or trimethoxybenzoate. Under these conditions acetate was excreted, indicating the function of carbon dioxide as electron acceptor in a homoacetogenic process. A growth-promoting effect of pyrophosphate added to the medium of Desulfotomaculum species was not observed. The results show a high catabolic and anabolic versatility among Desulfotomaculum species, and indicate that electron transport to sulfate can be the sole energy conserving process in this genus.