Evidence for the LH-releasing pathway of seminal plasma NGF in male camelids
Evidence for the LH-releasing pathway of seminal plasma NGF in male camelids
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DOI：
10.1016/j.theriogenology.2021.01.014
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发表时间：
2021-02-12
期刊：
影响因子：
2.8
通讯作者：
Adams, Gregg P.
中科院分区：
文献类型：
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作者：
Carrasco, Rodrigo A.;Pezo, Sergio;Adams, Gregg P.
在雌性骆驼中，全身给予NGF可诱导排卵前LH峰，导致排卵，但精液中NGF对雄性骆驼的影响尚不清楚。在本研究中，我们测试的假设，LH-释放途径的神经生长因子存在于雄性骆驼。实验1中，雄性美洲驼和羊驼分别用NGF或GnRH处理（n = 2头美洲驼和3头羊驼），并在处理前1 h至处理后3 h采集血样。在GnRH和NGF治疗组中，治疗后血浆LH浓度均以类似浪涌的方式增加，但在NGF治疗组中，浓度最高可达2.5倍，并持续升高至少2小时（治疗与时间相互作用，P = 0.01）。在实验2中，我们评估了LH和睾酮对NGF与盐水处理的反应（n = 3头美洲驼和3头羊驼）。促黄体生成素对NGF的反应与实验1相似，治疗后2、4、6 h，NGF组血浆睾酮浓度高于生理盐水组（P < 0.05）。结果支持这一假设，LH释放途径的NGF存在于男性南美骆驼。LH对NGF的反应维持了美洲驼循环中的睾酮浓度，表明NGF在睾酮分泌中具有中等作用。(C)2021爱思唯尔公司All rights reserved.
In the female camelid, systemic administration of NGF induces a preovulatory LH surge that results in ovulation, but the effects of seminal NGF in the male are unknown. In the present study, we tested the hypothesis that the LH-releasing pathway of NGF is present in male camelids. In Experiment 1, male llamas and alpacas were treated with NGF or GnRH (n = 2 llamas and 3 alpacas) and blood samples were collected from 1 h before to 3 h after treatment. Plasma LH concentrations increased after treatment in a surge-like fashion in both GnRH- and NGF-treated groups, but concentrations reached a maximum 2.5 times higher and remained elevated for at least 2 h longer in the NGF-treated group (treatment-by-time interaction, P = 0.01). In Experiment 2, we evaluated the LH and testosterone response to NGF vs saline treatment (n = 3 llamas and 3 alpacas). The LH response to NGF was similar to that in Experiment 1, and plasma testosterone concentrations were higher in the NGF group than in the saline group at 2, 4 and 6 h after treatment (P < 0.05). Results support the hypothesis that the LH-releasing pathway for NGF exists in male South American camelids. The LH response to NGF sustained circulating testosterone concentrations in llamas, suggesting a moderate role of NGF in testosterone secretion. (C) 2021 Elsevier Inc. All rights reserved.