Diversity, enumeration, and isolation of Arcobacter spp. in the giant abalone, Haliotis gigantea
Diversity, enumeration, and isolation of Arcobacter spp. in the giant abalone, Haliotis gigantea
复制标题
弓形杆菌属的多样性、计数和分离。
DOI：
10.1002/mbo3.890
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发表时间：
2019
期刊：
影响因子：
3.4
通讯作者：
Tanaka Reiji
中科院分区：
文献类型：
--
作者：
Mizutani Yukino;Iehata Shunpei;Mori Tetsushi;Oh Ryota;Fukuzaki Satoshi;Tanaka Reiji
在双壳类动物中经常检出和分离出弧菌，但在鲍鱼这种重要的渔业资源中，弧菌属的信息却很少。本研究旨在利用arcobacter特异性克隆文库和荧光原位杂交（FISH）等分子方法，研究日本巨鲍鱼（Haliotis gigantea）中arcobacter属细菌的遗传多样性和丰度。此外，我们试图分离检测到的arcobacterspecies。从大肠杆菌特异性克隆文库中获得了12个基因型的克隆。这些序列与其他已知的包括致病性arcobacterspp、A。butzleri， A. skirrowii,andA。嗜冷菌，通常从双壳类动物中分离或检测到。从FISH分析中，我们观察到ARC94F阳性细胞（假定为beArcobacter）占所有EUB338阳性细胞的6.96±0.72%。采用培养法从鲍鱼中分离出3种基因型的弧菌。其中一个基因型与marinus arcobacter 16S rRNA基因的相似性为99.2% ~ 100.0%，而其他基因型与其他arcobacterspecies的相似性仅为93.3% ~ 94.3%。这些数据表明鲍鱼携带杆状杆菌是一种常见的细菌属，包括未培养的种类。
Arcobacterhave been frequently detected in and isolated from bivalves, but there is very little information on the genusArcobacterin the abalone, an important fishery resource. This study aimed to investigate the genetic diversity and abundance of bacteria from the genusArcobacterin the Japanese giant abalone,Haliotis gigantea, using molecular methods such asArcobacter‐specific clone libraries and fluorescence in situ hybridization (FISH). Furthermore, we attempted to isolate theArcobacterspecies detected. Twelve genotypes of clones were obtained fromArcobacter‐specific clone libraries. These sequences are not classified with any other knownArcobacterspecies including pathogenicArcobacterspp.,A. butzleri, A. skirrowii,andA.cryaerophilus, commonly isolated or detected from bivalves. From the FISH analysis, we observed that ARC94F‐positive cells, presumed to beArcobacter,accounted for 6.96 ± 0.72% of all EUB338‐positive cells. In the culture method, three genotypes ofArcobacterwere isolated from abalones. One genotype had a similarity of 99.2%–100.0% to the 16S rRNA gene ofArcobacter marinus, while the others showed only 93.3%–94.3% similarity to otherArcobacterspecies. These data indicate that abalones carryArcobacteras a common bacterial genus which includes uncultured species.