CELL-ENVELOPE ASSOCIATIONS OF AQUASPIRILLUM-SERPENS FLAGELLA
CELL-ENVELOPE ASSOCIATIONS OF AQUASPIRILLUM-SERPENS FLAGELLA
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DOI：
10.1128/jb.136.3.1037-1049.1978
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
MURRAY, RGE
中科院分区：
文献类型：
--
作者：
COULTON, JW;MURRAY, RGE
与革兰氏阴性菌A的鞭毛基底复合体相关的细胞包膜的特定区域。通过研究每个包膜层（外膜、粘肽和质膜）来鉴定（螺菌）蛇形物。鞭毛插入位点周围的外膜由同心的膜环和由紧密设置的项圈包围的中央穿孔区分。外膜和分离的粘肽层中的穿孔的大小容纳基底复合物的中心杆，但小于L [含脂多糖的膜]或P [肽聚糖层]盘。复合物的P盘可能位于粘肽和外膜之间。完整的，球形或自溶制剂的EM没有充分解决的基础复杂的内部对磁盘的位置。冷冻蚀刻显示分化的质膜内，似乎是相关的基础复杂。凸起的断裂面显示凹陷，其被解释为被一组均匀间隔的大分子螺柱包围的圆盘的压痕，并且包含被解释为中心杆的中心塞。在薄切片中，在质膜的细胞质侧观察到与鞭毛器相关的泡。将鞭毛基底复合体的尺寸和细胞被膜层的间距叠加，并使用L盘在外膜内的位置作为参考，表明S [suprammembrane]盘可能在质膜内，M [cellular membrane]在质膜下。一个尝试性的模型开发与大肠杆菌基础复合物的结构的基础上进行比较。
Specific regions of the cell envelope associated with the flagellar basal complex of the gram-negative bacterium A. (Spirillum) serpens were identified by studying each of the envelope layers: outer membrane, mucopeptide and plasma membrane. The outer membrane around the flagella insertion site was differentiated by concentric membrane rings and central perforations surrounded by a closely set collar. The perforations in both the outer membrane and the isolated mucopeptide layer were of a size accomodating the central rod of the basal complex but smaller than either the L [lipopolysaccharide-containing membrane] or the P [peptidoglycan layer] disks. The P disk of the complex may lie between the mucopeptide and the outer membrane. EM of intact, spheroplasted or autolyzed preparations did not adequately resolve the location of the inner pair of disks of the basal complex. Freeze-etching revealed differentiation within the plasma membrane that appeared to be related to the basal complex. The convex fracture face showed depressions which are interpreted as impressions of a disk surrounded by a set of evenly spaced macromolecular studs and containing a central plug interpreted as the central rod. In thin sections, blebs, which appear to be associated with the flagellar apparatus, were seen on the cytoplasmic side of the plasma membrane. Superimposing the dimensions of the flagellar basal complex and the spacings of the cell envelope layers and using the position of the L disk within the outer membrane for reference, showed that the S [supramembrane] disk might be within and the M [cytoplasmic membrane] beneath the plasma membrane. A tentative model was developed for comparison with that based on the structure of the Escherichia coli basal complex.