Characterization of integrin expression in a well differentiated endometrial adenocarcinoma cell line (Ishikawa)
Characterization of integrin expression in a well differentiated endometrial adenocarcinoma cell line (Ishikawa)
复制标题
DOI：
10.1210/jc.82.1.136
复制
发表时间：
1997-01-01
影响因子：
5.8
通讯作者：
Lessey, BA
中科院分区：
文献类型：
--
作者：
Castelbaum, AJ;Ying, L;Lessey, BA
最近已经建立了正常子宫内膜中组成型和周期依赖性整合素的模式，表明细胞粘附分子在子宫内膜容受性和着床中的作用。目前，很少，如果有的话，模型存在的研究人类子宫内膜整合素和他们的作用，建立接受性子宫内膜表型。石川细胞系维持功能性雌激素受体和孕激素受体。这些细胞中的孕酮受体可通过雌二醇引发诱导，并通过孕酮处理下调。在本研究中，整合素的表达模式，在这个分化良好的子宫内膜细胞系相比，在正常子宫内膜上皮细胞使用免疫组化和现在的细胞计数，并证实免疫沉淀，Western免疫印迹，FCR。与正常子宫内膜上皮一样，石川细胞维持α（2）β（1）、α（3）β（1）、α（6）β（1）的组成型表达。PCR证实了每一种的预期大小片段，尽管注意到了α（2）亚基可变剪接形式的证据。雌二醇预处理的细胞导致α（1）β（1）胶原-层粘连蛋白受体表达增加和α（V）β（3）玻连蛋白受体（正常子宫内膜上皮细胞表达的两种周期依赖性整合素）的抑制。三个数据支持使用石川细胞作为一个很好的模型来研究子宫内膜整合素的调节，并推进我们对子宫内膜介导的着床事件的理解。
The pattern of constitutive and cycle-dependent integrins in normal endometrium has recently been established, suggesting a role for cell adhesion molecules in endometrial receptivity and implantation. Currently few, if any, models exist for the study of human endometrial integrins and their role in establishment of the receptive endometrial phenotype. The Ishikawa cell line maintains functional estrogen receptors and progesterone receptors. The progesterone receptors in these cells are inducible by priming with estradiol and down-regulated by treatment with progesterone. In the present study, the pattern of integrin expression in this well differentiated endometrial cell line is compared to that in normal endometrial epithelium using immunohistochemistry and now cytometry and is confirmed by immunoprecipitation, Western immunoblot, and FCR. Like normal endometrial epithelium, Ishikawa cells maintain constitutive expression of alpha(2) beta(1), alpha(3) beta(1), alpha(6) beta(1). PCR demonstrates the expected size fragments of each, although evidence for alternatively spliced forms of the alpha(2)-subunit was noted. Progesterone treatment of estradiol-primed cells resulted in increased expression of the alpha(1) beta(1) collagen-laminin receptor and suppression of the alpha(V) beta(3) vitronectin receptor, two of the cycle-dependent integrins expressed by normal endometrial epithelium. Three data support the use of Ishikawa cells as an excellent model to study the regulation endometrial integrins and advance our understanding of hormonally mediated events surrounding implantation.