RNA interference directed against poly(ADP-Ribose) polymerase 1 efficiently suppresses human immunodeficiency virus type 1 replication in human cells
RNA interference directed against poly(ADP-Ribose) polymerase 1 efficiently suppresses human immunodeficiency virus type 1 replication in human cells
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DOI：
10.1128/jvi.78.16.8931-8934.2004
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发表时间：
2004-08-01
影响因子：
5.4
通讯作者：
Yoshihara, K
中科院分区：
文献类型：
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作者：
Kameoka, M;Nukuzuma, S;Yoshihara, K
我们建立了针对聚（ADP-核糖）聚合酶1（PARP-1）的小干扰RNA（siRNA），可有效降低两种人类细胞系中PARP-1的表达。针对PARP-1建立的siRNA显著抑制人类免疫缺陷病毒1型（HIV-1）复制，以及整合的HIV-1长末端重复启动子的激活。这些结果表明，PARP-1是HIV-1在人类细胞中有效复制所必需的。我们建议PARP-1可能作为RNA干扰介导的基因沉默的细胞靶点，以抑制HIV-1的复制。
We established small interfering RNA (siRNA) directed against poly(ADP-ribose) polymerase 1 (PARP-1) that effectively reduces the expression of PARP-1 in two human cell lines. Established siRNA against PARP-1 significantly suppressed human immunodeficiency virus type 1 (HIV-1) replication, as well as the activation of the integrated HIV-1 long terminal repeat promoter. These results indicate that PARP-1 is required for efficient HIV-1 replication in human cells. We propose that PARP-1 may serve as a cellular target for RNA interference-mediated gene silencing to inhibit HIV-1 replication.