Chromium and vanadate combination increases insulin-induced glucose uptake by 3T3-L1 adipocytes.
Chromium and vanadate combination increases insulin-induced glucose uptake by 3T3-L1 adipocytes.
复制标题
铬和钒酸盐组合可增加 3T3-L1 脂肪细胞胰岛素诱导的葡萄糖摄取。
DOI：
10.1016/j.bbrc.2006.06.154
复制
发表时间：
2006
影响因子：
3.1
通讯作者：
Wang,Hong
中科院分区：
文献类型：
--
作者：
Brautigan,DavidL;Kruszewski,Allison;Wang,Hong
Insulin activates signaling pathways in target tissues through the insulin receptor and Tyr phosphorylation of intracellular proteins. Vanadate mimics insulin and enhances its actions through inhibition of protein Tyr phosphatases. Chromium is a micronutrient that enhances insulin action to normalize blood glucose, but the mechanism is not understood. Here we show that either vanadate or chromium stimulates Tyr phosphorylation of insulin receptor in mouse 3T3-L1 adipocytes compared to insulin alone, but a combination of vanadate and chromium is not additive. Phosphorylation of MAPK or 4E-BP1 as markers for insulin signaling is stimulated by vanadate plus insulin, and chromium does not enhance the effects. Vanadate robustly activates glucose uptake by 3T3-L1 adipocytes even without added insulin and increases insulin-stimulated glucose uptake. Chromium pretreatment of adipocytes slightly enhances glucose uptake in response to insulin, but significantly increases glucose uptake above that induced by insulin plus vanadate. These data show that chromium enhances glucose uptake even when Tyr phosphorylation levels are elevated by vanadate plus insulin, suggesting separate mechanisms of action for vanadate and chromium.
