Hepatocellular adenoma subtype classification using molecular markers and lmmunohistochemistry
Hepatocellular adenoma subtype classification using molecular markers and lmmunohistochemistry
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DOI：
10.1002/hep.21743
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发表时间：
2007-09-01
期刊：
影响因子：
13.5
通讯作者：
Zucman-Rossi, Jessica
中科院分区：
文献类型：
--
作者：
Bioulac-Sage, Paulette;Rebouissou, Sandra;Zucman-Rossi, Jessica
具有激活的 P-连环蛋白的肝细胞腺瘤 (HCA) 具有很高的恶性转化风险。为了进行稳健的常规诊断以进行 HCA 亚型分类，我们搜索了新的有用标记。我们通过定量逆转录聚合酶链反应 QRT-PCR 和免疫组织化学分析了候选基因的表达，以根据肝细胞核因子 1 α HNF1 α a) 和 P-连环蛋白突变以及炎症表型验证其特异性和敏感性。定量 RT-PCR 显示 FABP1（肝脂肪酸结合蛋白）和 UGT2B7 在 HNF1 α 失活的 HCA 中下调（P
Hepatocellular adenomas (HCA) with activated P-catenin present a high risk of malignant transformation. To permit robust routine diagnosis to allow for HCA subtype classification, we searched new useful markers. We analyzed the expression of candidate genes by quantitative reverse transcription polymerase chain reaction QRT-PCR followed by inummohistochemistry to validate their specificity and sensitivity according to hepatocyte nuclear factor 1 alpha HNF1 alpha a) and P-catenin mutations as well as inflammatory phenotype. Quantitative RT-PCR showed that FABP1 (liver fatty acid binding protein) and UGT2B7were downregulated in HNF1 alpha-inactivated HCA (P